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Anti-phospho-MAP Kinase 1/2 (Erk1/2)(Thr185/Tyr187), clone AW39
Recommended Replacement for: 05-797
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PRODUCT FAMILY INFORMATION
Erk (MAP) Kinase Antibodies
Millipore’s Erk Antibodies demonstrate specificity against Erk kinases, p44 and p42 MAP kinases. See below for related products, including proteins & kits based on the expertise of Upstate & Chemicon.
Millipore’s Anti- Erk Antibodies demonstrates specificity against Erk. See below for data, references and related products for Erk. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
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MAPK Antibodies, Proteins, Inhibitors, & Assays
Offering hundreds of premium quality antibodies, enzymes, activity assays, and small molecule inhibitors for MAPK signaling research
EMD Millipore offers hundreds of premium quality antibodies, enzymes, activity assays, and small molecule inhibitors for MAPK signaling research. All EMD Millipore products are based on the expertise of Upstate, Chemicon, and Calbiochem.
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Species Reactivity Key Applications Host Format Antibody Type
H, R Mplex, WB Rabbit null Monoclonal Antibody
Description:
Anti-phospho-MAP Kinase 1/2 (Erk1/2)(Thr185/Tyr187) Antibody, clone AW39
Replaces:
05-797
Promotional Text:
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes phosphorylated MAP Kinase 1 and 2 (Erk 1&2).
Molecular Weight:
MAPK1 (~44 kDa) and MAPK2 (~42 kDa).
Immunogen:
KLH-conjugated, synthetic peptide corresponding to amino acids surrounding the pTEpY motif of human phospho-MAP Kinase 1/2 (Erk1/2), in which the Thr and Tyr residues are phosphorylated. The immunizing sequence is highly conserved among species.
Modifications:
Phosphorylation
Clone:
Aw39
Isotype:
IgG
Background Information:
Erk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs) and/or integrin stimulation. This activates the Ras-Raf-MEK,-Erk pathway that results in MEK, a dual kinase (a Ser/Thr and a Tyr kinase), phosphorylation and activation of ERK1/2 (p44/044) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 and Erk2, respectively).
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Species Reactivity:
Human
Rat
Species Reactivity Note:
Human and rat. Predicted to cross-react with many species based on sequence homology.
Application Notes:
Western Blot Analysis:
A 1:2,000-1:8,000 dilution of this lot detected phospho-MAP Kinase 1/2 (Erk1/2) in RIPA lysates from NGF stimulated PC-12 cells (Figure A).
Beadlyte® Phospho-Specificity Assay:
1:500-1:4,000 dilutions of a previous lot detected active but not unactive MAP Kinase/Erk2 conjugated to Luminex® microspheres. (Figure B).
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Control:
RIPA cell lysate from NGF stimulated PC-12 cells
Quality Assurance:
Routinely evaluated by immunoblot on RIPA lysates from NGF stimulated |