|
Anti-trimethyl-Histone H3 (Lys36), clone MC86
Close
REFERENCES
Heterochromatin protein 1a stimulates histone H3 lysine 36 demethylation by the Drosophila KDM4A demethylase.
Lin, Chia-Hui, et al. (2008) Mol. Cell, 32: 696-706 (2008)
Global patterns of histone modifications.
Rando, Oliver J (2007) Curr. Opin. Genet. Dev., 17: 94-9 (2007)
Proteome-wide analysis in Saccharomyces cerevisiae identifies several PHD fingers as novel direct and selective binding modules of histone H3 methylated at either lysine 4 or lysine 36.
Shi, Xiaobing, et al. (2007) J. Biol. Chem., 282: 2450-5 (2007)
A chromatin landmark and transcription initiation at most promoters in human cells.
Guenther, Matthew G, et al. (2007) Cell, 130: 77-88 (2007)
Species Reactivity Key Applications Host Format Antibody Type
Ch DB, PIA, WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-trimethyl-Histone H3 (Lys36) Antibody, clone MC86, rabbit monoclonal
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes trimethyl-histone H3 (Lys36), Mr ~17 kDa. A non-specific protein may also be detected in some cell lysates, Mr ~78 kDa.
Molecular Weight:
~17kDa
Immunogen:
KLH-conjugated, synthetic peptide containing the sequence …GVme3KKP…, in which me3K corresponds to trimethyl lysine 36 of human histone H3.
Modifications:
Methylation
Clone:
MC86
Isotype:
IgG
Background Information:
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Methylation of histone H3 on Lys36 (H3K36me2/3) is tightly associated with actively transcribed genes, and this modification is found primarily within the coding region, suggesting H3K36 methylation is necessary for efficient RNA polymerase II elongation and processivity.
View All »
Species Reactivity:
Chicken
Species Reactivity Note:
Broad species cross-reactivity is expected.
Application Notes:
Peptide Inhibition Analysis:
1 μM of histone H3 peptides containing trimethyl-lysine 36 abolished detection of histone H3 by anti-trimethyl-Histone H3 (Lys36) in immunoblot analysis of chicken erythrocyte core histones (Lane 5) in a previous lot. Signal reduction was also observed with 1 μM of a histone H3 peptide containing dimethyl-lysine 36 (Figure A).
Peptide Dot Blot Analysis:
A 1:1000 dilution of a previous lot only detected peptides containing the trimethyl-lysine 36 modification. (Figure B, page two).
View All »
Control:
Chicken Core Histones
Quality Assurance:
Routinely evaluated by western blot analysis on chicken core Histones.
estern Blot Analysis:
A 1:1000-1:2000 dilution of this lot detected trimethyl histone H3 (Lys36) in chicken Core Histone |