|
Anti-phospho-PKD (Ser916), clone MC29
Recommended Replacement for: 05-787
Close
REFERENCES
Protein kinase D1 mediates stimulation of DNA synthesis and proliferation in intestinal epithelial IEC-18 cells and in mouse intestinal crypts.
James Sinnett-Smith,Nora Rozengurt,Robert Kui,Carlos Huang,Enrique Rozengurt (2011) The Journal of biological chemistry.286
Species Reactivity Key Applications Host Format Antibody Type
H, M WB Rabbit null Monoclonal Antibody
Description:
Anti-phospho-PKD (Ser916) Antibody, clone MC29 | 04-787
Replaces:
05-787
Promotional Text:
Special Offer on Antibodies! Click Here!
Trade Name:
Upstate (Millipore)
Specificity:
phospho-PKD
Molecular Weight:
~105-110kDa
Immunogen:
peptide containing RVpSIL in which pS corresponds to phospho-serine 916 of mouse PKD
Modifications:
Phosphorylation
Clone:
MC29
Isotype:
IgG
Background Information:
There are two PKD isoforms (PKD1 and PKD2) belonging to the polycystin family. These proteins are involved in protein-protein and protein-carbohydrate adhesion. The two isoforms are known to interact, and also thought to be ion channel regulators. Defects in PKD1 have been identified as the cause of autosomal dominant polycystic kidney disease type I (ADPKD).
View All »
Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human. Predicted to cross-react with mouse based on sequence homology.
Application Notes:
Western Blot Analysis:
1:500-1:2000 of this lot detected PKD in RIPA lysates from HEK293 cells stimulated with phorbol 12,13-dibutyrate (PDBu) for 10 minutes at 50 ng/mL.
Control:
RIPA lysates from HEK293 cells
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from HEK293 cells stimulated with phorbol 12,13-dibutyrate
Presentation:
Cultured supernantant in 0.05% sodium azide
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
UniProt Number:
Q15139
Entrez Gene Number:
NM_002742
Gene Symbol:
PRKD1
PKD
PKCM
nPKC-mu
PRKCM
PKC-mu
nPKC-D1
PKC-MU
PKD1
View All »
Alternate Names:
Protein kinase C mu type
Protein kinase D
polycystic kidney disease 1 (autosomal dominant)
polycystic kidney disease-associated protein
polycystin 1
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Entrez Gene Summary:
Members of the protein kinase C (PKC) family function in many extracellular receptor-mediated signal transduction pathways. See PRKCA (MIM 176960) for further background information. The PRKCM gene encodes a cytosolic serine-threonine kinase that binds to the trans-Golgi network and regulates the fission of transport carriers specifically destined to the cell surface.[supplied by OMIM]
View All »
UniProt Summary:
FUNCTION: SwissProt: Q15139 # This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
SIZE: 912 amino acids; 101888 Da
SUBUNIT: Interacts via its N-terminal region with CENTA1.
|