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Anti-IRS1, clone AW58
Recommended Replacement for: 05-784
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REFERENCES
Protein kinase C-zeta phosphorylates insulin receptor substrate-1 and impairs its ability to activate phosphatidylinositol 3-kinase in response to insulin.
Ravichandran, L V, et al. (2001) J. Biol. Chem., 276: 3543-9 (2001)
Components of signaling pathways for insulin and insulin-like growth factor-I in muscle myoblasts and myotubes.
Lamphere, L and Lienhard, G E (1992) Endocrinology, 131: 2196-202 (1992)
Mammalian target of rapamycin pathway regulates insulin signaling via subcellular redistribution of insulin receptor substrate 1 and integrates nutritional signals and metabolic signals of insulin
Takano, A., et al (2001) Mol Cell Biol, 21:5050-62 (2001)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R IP, WB Rabbit null Monoclonal Antibody
Description:
Anti-IRS1 Antibody, clone AW58 | 04-784
Replaces:
05-784
Promotional Text:
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Trade Name:
Upstate (Millipore)
Specificity:
IRS1
Molecular Weight:
Mr ~145-165kDa
Immunogen:
Peptide corresponding to carboxy-terminal 14 amino acids (C-YASINFQKQPEDRQ) of rat liver IRS1
Clone:
AW58
Isotype:
IgG
Background Information:
Insulin Receptor substrate proteins are the effectors of both Insulin and IGF-initiated signaling. They share PH and PTB domains near their n-termini, and multiple Tyr phosphorylation motifs in their c-terminal regions. Proteins which bind to tyrosine-phosphorylated IRS-proteins include PI3 Kinase p85, GRB2, SHP2, Nck, Crk, and Fyn. IRS1 appears to be principally involved in IGF-signaling and cytoskeletal growth. IRS2 appears to be the critical mediator of Insulin signaling, as genetic ablation results in type II diabetes. IRS3 is expressed primarily in adipocytes and is an unusually potent activator of PI3 Kinase. IRS4 lacks the tyrosine residues which in the other IRS-proteins binds to SHP2.
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Species Reactivity:
Human
Mouse
Rat
Application Notes:
Immunoblot Analysis: 1:1,000 to 1:4,000 dilutions of this lot detected IRS1 in RIPA lysates from 3T3/A31 mouse fibroblasts.
Immunoprecipitation: 5-10μl of this lot immunoprecipitated IRS1 from 500μg of 3T3/A31 RIPA lysate.
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Quality Assurance:
routinely evaluated by immunoblot on in RIPA lysates from 3T3/A31 mouse fibroblasts
Presentation:
Cultured supernantant in 0.05% sodium azide
Storage Conditions:
1 year at -20°C from date of shipment
UniProt Number:
P35568
Entrez Gene Number:
NM_005544
Gene Symbol:
IRS1
IRS-1
HIRS-1
Alternate Names:
insulin receptor substrate 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P35568 # May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).
SIZE: 1242 amino acids; 131591 Da
SUBUNIT: |