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Anti-phospho-Histone H3 (Thr11) Antibody, clone MC83
Recommended Replacement for: 05-789
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PRODUCT FAMILY INFORMATION
Histone H3 Antibodies, Proteins and Kits
Millipore’s Histone H3 antibodies demonstrate specificity against histone H3. See below for acetyl-, methyl-, phospho- histone H3 Antibodies and Proteins, based on the expertise of Upstate & Chemicon.
Millipore’s Anti-Histone H3 Antibody demonstrates specificity against Histone H3. See below for data, references and related products for Histone H3. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
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Species Reactivity Key Applications Host Format Antibody Type
H WB, PIA, Mplex Rabbit null Monoclonal Antibody
Description:
Anti-phospho-Histone H3 (Thr11) Antibody, clone MC83 | 04-789
Replaces:
05-789
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes histone H3 phosphorylated at Thr11, Mr 17kDa.
Molecular Weight:
17kDa
Immunogen:
Peptide containing ...KS[pT]G..., in which pT corresponds to phospho-threonine at position 11 of human histone H3
Modifications:
Phosphorylation
Clone:
MC83
Isotype:
IgG
Background Information:
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications, which include acetylation, phosphorylation, methylation, and ubiquitin-ation, occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. In mammals, phosphorylation of Thr11 modification is restricted to the centromeric region while in plant cells, phosphorylation of Thr11 was distributed along the entire length of condensed chromosomes. Mitosis-specific phosphorylation of histone H3 at Thr11 is mediated by Dlk/ZIP kinase.
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Species Reactivity:
Human
Species Reactivity Note:
Human. Broad species cross-reactivity is expected based on sequence homology. An additional unidentified band at ~40 kDa appears in some lysates.
Application Notes:
Immunoblot Analysis:
A 1:500-1:2000 dilution of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells treated with colcemid, but not unmodified recombinant histone H3 (Catalog # 14-494).
Western Blot Analysis:
A 1:5,000 dilution of a previous lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells treated with colcemid, but not unmodified recombinant histone H3 (Catalog # 14-494).
Multiplexing:
Beadlyte® Histone-Peptide Specificity Assay: 1:1,000-1:50,000 dilutions of a previous lot were incubated with histone H3 peptides containing various modifications conjugated to Luminex® microspheres. Only the peptide containing phospho-threonine 11 was
detected (Figure C).
Peptide Inhibition Analysis: 1 μM of histone H3 peptide containing phospho-threonine 11 abolished detection of histone H3 by anti-phospho-Histone H3 (Thr11), clone MC83 in immunoblot analysis of acid extracts from
colcemid treated HeLa cells (Figure D, lane 4).
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Control:
HeLa acid extracts
Quality Assurance:
Routinely evaluated by immunoblot analysis.
Presentation:
Cultured |