|
Anti-Ha-Ras, clone MC57
Recommended Replacement for: 05-775
Close
PRODUCT FAMILY INFORMATION
RAS Antibodies, Kits, Proteins & Plasmids
Millipore’s RAS antibodies, kits, and plasmids are high quality, validated tools for research. These are based on the expertise of Upstate & Chemicon. See below for Millipore’s Ras G-protein products.
Millipore’s RAS antibodies, kits, plasmids and proteins are high quality, validated tools for RAS research. See below for data and references for Millipore’s RAS products. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
» More…
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB Rabbit null Monoclonal Antibody
Description:
Anti-Ha-Ras Antibody, clone MC57 | 04-775
Replaces:
05-775
Promotional Text:
Special Offer on Antibodies! Click Here!
Specificity:
Recognizes Ha-Ras, Mr ~21 kDa. Does not cross react with Cdc42, Rho or Rac1.
Molecular Weight:
~21 kDa
Immunogen:
GST fusion protein corresponding to full length human Ha-Ras. Clone MC57.
Clone:
MC57
Isotype:
IgG
Background Information:
Ras proteins are small GTP-binding proteins which, unlike the heterotrimeric G-proteins, contain all GTPase and effector functions within a single polypeptide. At least three isoforms of Ras exist, Ki-Ras, Ha-Ras and N-Ras, with distinct expression patterns but similar signaling activity. Ras is palmitoylated and farnesylated at the carboxy terminus, anchoring it in the membrane. In resting cells, Ras is loaded with GDP, and is activated subsequent to growth factor stimulation of receptors, which recruit Ras Guanine nucleotide Exchange Factors to the plane of the membrane. Proximity of exchange factors to the Ras proteins causes release of GDP, and its replacement by GTP. In its GTP-bound form, Ras binds several proteins, including Raf, RalGDS and PI3 Kinase. Inactivation of Ras occurs by GTP hydrolysis, which is greatly accelerated by RasGAP or NF-1, two known Ras GTPase Activating Proteins. It is possible to assay for Ras activation by incubation of lysates with the Ras-binding domain of Raf-1, which selectively binds to Ras:GTP.
View All »
Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human. Predicted to cross-react with mouse and rat based on sequence homology.
Control:
Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Quality Assurance:
Western Blot Analysis: A 1:1000 dilution of this antibody detected Ha-Ras in RIPA lysates from A431 cells.
Presentation:
Cultured supernantant containing 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P01112
Entrez Gene Number:
NP_005334.1
Gene Symbol:
C-BAS/HAS
C-H-RAS
C-HA-RAS1
CTLO
H-RASIDX
H-Ras-1
HAMSV
HRAS1
Ha-Ras
K-RAS
N-RAS
OTTHUMP00000162769
OTTHUMP00000166053
OTTHUMP00000166055
RASH1
c-H-ras
p21ras
View All »
Alternate Names:
GTP- and GDP-binding peptide B
GTPase HRas
Ha-Ras1 proto-oncoprotein
Ras family sm |