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Anti-phospho-MYPT1 (Thr850),
clone SA19
Recommended Replacement for: 05-773
Species Reactivity Key Applications Host Format Antibody Type
Ch, H, M, R WB Rabbit null Monoclonal Antibody
Description:
Anti-phospho-MYPT1 (Thr850) Antibody,
clone SA19 | 04-773
Replaces:
05-773
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Specificity:
Recognizes MYPT1 phosphorylated on Thr850, doublet at Mr ~130 kDa. A non-specific protein was also detected, Mr ~55-60 kDa.
Molecular Weight:
Doublet at ~130 kDa
Epitope:
pThr850(Ch) or pThr853(H)
Immunogen:
KLH-conjugated, synthetic peptide containing the sequence …RS[pT]GV…, in which [pT] corresponds to phosphorylated threonine at amino acid residue 850 of chicken myosin phosphatase target subunit 1 (MYPT1). Corresponds to Thr853 in human MYPT1.
Modifications:
Phosphorylation
Clone:
SA19
Isotype:
IgG
Background Information:
Phosphorylation of myosin is crucial to smooth muscle contraction. The degree of myosin phosphorylation is dependent in part by the activity of myosin phosphatase. Recent findings suggest that this enzyme can be regulated, which has lead to interest in characterizing this phosphatase. Myosin phosphatase seems to be comprised of three subunits (catalytic subunit of type 1 phosphatase-δ (PP1) and two non-catalytic subunits, large and small (M20)). Studies suggest that the large subunit is a targeting subunit and is termed myosin phosphatase target subunit (MYPT). MYPT has been shown to be a substrate for the Rho-associated kinase (ROK). ROK mediates an agonist-induced increase in myosin phosphorylation and force by inhibiting myosin light chain phosphatase activity through phosphorylation of MYPT.
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Species Reactivity:
Chicken
Human
Mouse
Rat
Species Reactivity Note:
Human, Chicken.
Predicted to cross-react with mouse and rat.
Control:
HEK293 cell lysate phosphorylated by ROKα/ROCKII.
Quality Assurance:
Westerin Blot Analysis: A 1:500-1:1000 dilution of this antibody detected MYPT1 in RIPA lysates from HEK293 cells phosphorylated by preincubation with ROKα/ROCK-II, active (Catalog # 14-338).
Presentation:
Cultured supernantant containing 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
O14974
Entrez Gene Number:
NP_002471.1
Gene Symbol:
MBS
MGC133042
MYPT1
Alternate Names:
Myosin phosphatase target subunit 1
Myosin phosphatase-targeting subunit 1
Protein phosphatase myosin-binding subunit
myosin phosphatase, target subunit 1
protein phosphatase 1, regulatory (inhibitor) subunit 12A
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulat |