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Anti-Src Antibody, CT, clone NL19 | 04-772
Recommended Replacement for: 05-772
Species Reactivity Key Applications Host Format Antibody Type
H, M, R IP, WB Rabbit null Monoclonal Antibody
Description:
Anti-Src Antibody, CT, clone NL19 | 04-772
Replaces:
05-772
Promotional Text:
Special Offer on Antibodies! Click Here!
Trade Name:
Upstate (Millipore)
Specificity:
Src-tyrosine kinase family
Molecular Weight:
~53-62kDa
Immunogen:
KLH conjugated synthetic peptide (CKVADFGLARLIEDNEYTARQGA) of Src (avian sarcoma virus), residues 400-422. The immunogen sequence shares 22/23 amino acids with Yes and Fyn, and 21/23 amino acids with Lyn and Hck.
Clone:
NL19
Isotype:
IgG
Background Information:
SRC-1 is a member of a gene family that functions in the full transcriptional activation of the steroid hormone receptor superfamily and contains intrinsic histone acetyltransferase activity. SRC-1 has a molecular weight of 160 kDa and has a basic helix-loop-helix (bHLH)-PAS domain.
Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Predicted to cross react with frog and chicken. The immunogen sequence shares 22/23 amino acids with Yes and Fyn, and 21/23 amino acids with Lyn and Hck.
Application Notes:
Western Blot Analysis: A 1:2000 to 1:8000 dilution of this lot detected Src in RIPA whole cell lysates from human A431 cells. Immunoprecipitation: 5-20 μL of a previous lot immunoprecipitated Src from 500 μg of A431 RIPA lysate.
Control:
RIPA whole cell lysates from human A431, Jurkat cells, mouse 3T3/A31 cells or rat PC-12 cells Included Positive Antigen Control: Catalog # 12-301, Non-Stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Quality Assurance:
routinely evaluated by immunoblot on RIPA whole cell lysates from human A431 and Jurkat cells, mouse 3T3/A31 cells or rat PC-12 cells
Presentation:
Cultured supernantant in 0.05% sodium azide
Storage Conditions:
Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
UniProt Number:
P12931
Entrez Gene Number:
NM_005417
Gene Symbol:
SRC
c-src
p60-Src
SRC1
c-SRC
ASV
pp60c-src
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Alternate Names:
proto-oncogene tyrosine-protein kinase SRC
protooncogene SRC, Rous sarcoma
tyrosine kinase pp60c-src
tyrosine-protein kinase SRC-1
v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript varian |