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Anti-Akt2/PKBβ, clone AW114
Recommended Replacement for: 05-771
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, IP Rabbit null Monoclonal Antibody
Description:
Anti-Akt2/PKBβ Antibody, clone AW114 | 04-771
Replaces:
05-771
Promotional Text:
Special Offer on Antibodies! Click Here!
Specificity:
Recognizes Akt2/PKBβ, Mr ~60 kDa.
Molecular Weight:
~60 kDa
Epitope:
a.a. 455-469
Immunogen:
KLH-conjugated, synthetic peptide (CRYDSLGSLELDQRTH) corresponding to amino acids 455-469 of human Akt2/PKBβ, with an N-terminal cysteine added for conjugation purposes.
Clone:
AW114
Isotype:
IgG
Background Information:
Akt is the major known effector of the PI3 Kinase pathway. Generation of PIP3 results in the activation of PDK1, which phosphorylates Akt on Thr308, and another kinase complex, thought to be the mTORC2 complex (or possibly an intermediate kinase linking the two) that phosphorylates Akt on Ser473. These phosphorylations additively activate Akt Ser/Thr kinase activity, and the use of phosphorylation state-specific antibodies directed against either of these sites can imply Akt activation. Activation of Akt can be measured directly by immunoprecipitation followed by phosphorylation of a known substrate with radiolabeled ATP. Akt has been shown to phosphorylate over 70 substrates including TSC1&2 in the mTOR pathway, Bad on Ser136, GSK3 (which is inactivated by this phosphorylation), and the FoxO family of transcription factors (PRAS40, AS160).
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human and rat. Predicted to cross-react with mouse.
Control:
L6 cell lysate
Quality Assurance:
Western Blot Analysis: A 1:500 dilution of this antibody detected Akt2/PKBβ in HepG2 lysate.
Presentation:
Cultured supernantant containing 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P31751
Entrez Gene Number:
NP_001617.1
Gene Symbol:
EC 2.7.11.1
PKBB
PKBBETA
PRKBB
RAC-BETA
RAC-PK-beta
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Alternate Names:
AKT2 kinase
Murine thymoma viral (v-akt) homolog-2
PKB beta
Protein kinase Akt-2
Protein kinase B, beta
rac protein kinase beta
v-akt murine thymoma viral oncogene homolog 2
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins.
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