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Anti-trimethyl-Histone H3 (Lys4), clone MC315; 100 μL
Recommended Replacement for: 05-745
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REFERENCES
Endogenous mammalian histone H3.3 exhibits chromatin-related functions during development.
Bush, Kelly M, et al. (2013) Epigenetics Chromatin, 6: 7 (2013)
ATF4-dependent Regulation of the JMJD3 Gene during Amino Acid Deprivation Can Be Rescued in Atf4-deficient Cells by Inhibition of Deacetylation.
Jixiu Shan,Lingchen Fu,Mukundh N Balasubramanian,Tracy Anthony,Michael S Kilberg (2012) The Journal of biological chemistry.287
Overexpression of a histone H3K4 demethylase, JMJ15, accelerates flowering time in Arabidopsis.
Hongchun Yang,Huixian Mo,Di Fan,Ying Cao,Sujuan Cui,Ligeng Ma (2012) Plant cell reports.31
G9a/GLP histone lysine dimethyltransferase complex activity in the hippocampus and the entorhinal cortex is required for gene activation and silencing during memory consolidation.
Swati Gupta-Agarwal,Aimee V Franklin,Thomas Deramus,Muriah Wheelock,Robin L Davis,Lori L McMahon,Farah D Lubin (2012) The Journal of neuroscience : the official journal of the Society for Neuroscience.32
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Species Reactivity Key Applications Host Format Antibody Type
H WB, DB, Mplex, ChIP-seq Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315, rabbit monoclonal
Replaces:
05-745
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Trade Name:
Upstate (Millipore)
Specificity:
Histone H3 containing trimethyl-lysine 4 and, to a lesser extent, dimethyl-lysine 4. See COA for specificity data.
Molecular Weight:
17 kDa
Immunogen:
Peptide containing the sequence [RTtrimKQ] in which lysine 4 is trimethylated on human histone H3
Modifications:
Methylation
Clone:
MC315
Isotype:
IgG
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
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Species Reactivity:
Human
Application Notes:
WB, ChIP, BD, PIA
Control:
HeLa acid extract
Quality Assurance:
routinely evaluated by immunoblot on in acid extracted proteins from HeLa cells, but not recombinant unmethylated Histone H3 (Catalog #14-494)
Presentation:
Cultured supernantant in 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Q16695
Entrez Gene Number:
NM_003493
Gene Symbol:
HIST3H3
H3FT
MGC126886
H3t
MGC126888
H3.4
H3T
H3/g
H3/t
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Alternate Names:
H3K4me3
Histone H3 (tri methyl K4)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are n |