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Anti-phospho-Histone H3 (Thr3), clone JY325; 100 μg
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PRODUCT FAMILY INFORMATION
Histone H3 Antibodies, Proteins and Kits
Millipore’s Histone H3 antibodies demonstrate specificity against histone H3. See below for acetyl-, methyl-, phospho- histone H3 Antibodies and Proteins, based on the expertise of Upstate & Chemicon.
Millipore’s Anti-Histone H3 Antibody demonstrates specificity against Histone H3. See below for data, references and related products for Histone H3. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
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Species Reactivity Key Applications Host Format Antibody Type
H WB, DB, Mplex, PIA Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-phospho-Histone H3 (Thr3) Antibody, clone JY325, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Histone H3 phosphorylated at Threonine 3; cross-reactivity with phospho-histone H3 Thr22 detected in some applications
Molecular Weight:
17 kDa
Immunogen:
Peptide containing the sequence [RTtrimKQ] in which lysine 4 is trimethylated on human histone H3
Modifications:
Phosphorylation
Clone:
JY325
Isotype:
IgG
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
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Species Reactivity:
Human
Application Notes:
WB, ChIP, BD, PIA
Control:
Acid extracted proteins from mitotic HeLa cells (Catalog # 17-306) treated with colcemid
Quality Assurance:
routinely evaluated by immunoblot on acid extracted proteins from mitotic HeLa cells (Catalog #17-306) treated with colcemid, but not unmodified recombinant histone H3
Presentation:
Cultured supernantant in 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Q16695
Entrez Gene Number:
NM_003493
Gene Symbol:
HIST3H3
H3FT
MGC126886
H3t
MGC126888
H3.4
H3T
H3/g
H3/t
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Alternate Names:
H3T3P
Histone H3 (phospho T3)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Dot Blot
Multiplexing
Peptide Inhibition Assay
Entrez Gene Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chr |