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Anti-SHP-1/2, clone NL213
Recommended Replacement for: 05-742
Species Reactivity Key Applications Host Format Antibody Type
H, M IP, WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-SHP-1/2 Antibody, clone NL213, rabbit monoclonal
Replaces:
05-742
Promotional Text:
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes SHP-1 greater than SHP-2, Mr ~65 kDa
Molecular Weight:
~65kDa
Immunogen:
Full-length glutathione transferase SHP-1 fusion protein, purified from bacteria
Clone:
NL213
Isotype:
IgG
Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human and mouse. Others not tested.
Quality Assurance:
routinely evaluated by immunoblot in RIPA lysates from human A431 carcinoma cells
Presentation:
Cultured supernantant in 0.05% sodium azide
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
P29350
Entrez Gene Number:
NM_002831, NM_002834
Gene Symbol:
PTPN6
HCP
SH-PTP1
PTP-1C
SHP1
SHP-1L
PTP1C
HCPH
SHP-1
HPTP1C
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported.
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Key Applications:
Immunoprecipitation
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P29350 # Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
SIZE: 595 amino acids; 67561 Da
SUBUNIT: Monomer. Interacts with MTUS1 (By similarity). Binds PTPNS1, LILRB1 and LILRB2. Interacts with FCRL2, CD300LF and CD84.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus (By similarity). Note=In neurons, translocates into the nucleus after treatment with angiotensin II (By similarity).
TISSUE SPECIFICITY: Isoform 1 is expressed in hematopoietic cells while isoform 2 is expressed in non-hematopoietic cells.
PTM: Phosphorylated on serine an |