|
Anti-JAM1 (NT) Antibody, rabbit monoclonal, clone EP1042Y
Close
REFERENCES
Junctional adhesion molecule, a novel member of the immunoglobulin superfamily that distributes at intercellular junctions and modulates monocyte transmigration.
Martìn-Padura, I, et al. (1998) J. Cell Biol., 142: 117-27 (1998)
Identification and characterisation of human Junctional Adhesion Molecule (JAM)
Williams, L A, et al (1999) Mol Immunol, 36:1175-88 (1999)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-JAM1 (NT) Antibody, rabbit monoclonal, clone EP1042Y
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Mouse, Rat JAM1 (N-Term).
Immunogen:
A synthetic peptide corresponding to residues near the N-terminus of human JAM1.
Clone:
EP1042Y
Background Information:
Junctional Adhesion Molecules (JAMs) are components and regulators of the well-characterized epithelial and endothelial tight junction. JAM is selectively concentrated at intercellular junctions of endothelial and epithelial cells of different origins. Microscopy shows that JAM codistributes with tight junction components at the apical region of the intercellular cleft. A mAb directed to JAM (BV11) was found to inhibit spontaneous and chemokine-induced monocyte transmigration through an endothelial cell monolayer in vitro. Systemic treatment of mice with BV11 mAb blocked monocyte infiltration upon chemokine administration in subcutaneous air pouches (1). The human protein shares a highly conserved structure and sequence with the murine protein. However it is distinct in that it is constitutively expressed on circulating neutrophils, monocytes, platelets and lymphocyte subsets (2).
View All »
Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human, Mouse, Rat (positive control: Hela cell lysate)
Application Notes:
WB: 1:2000
IHC: 1:100 - 250 (Paraffin-embedded human liver)
Purification Method:
Protein A purfied
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
Q9Y624
Entrez Gene Number:
NM_144502.1
NM_144504.1
NM_016946.3
NM_144503.1
Gene Symbol:
F11R
JAM-A
PAM-1
JAMA
CD321
JAM
JAM1
JAM-1
JCAM
KAT
View All »
Alternate Names:
Junctional Adhesion Molecule-1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Immunohistochemistry (Paraffin)
Entrez Gene Summary:
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a l |