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Anti-Flotillin-1 Antibody, rabbit monoclonal, clone EP446Y
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REFERENCES
Glycosylphosphatidyl inositol-anchored proteins and fyn kinase assemble in noncaveolar plasma membrane microdomains defined by reggie-1 and -2.
Stuermer, C A, et al. (2001) Mol. Biol. Cell, 12: 3031-45 (2001)
The SPFH domain: implicated in regulating targeted protein turnover in stomatins and other membrane-associated proteins
Tavernarakis, N, et al (1999) Trends Biochem Sci, 24:425-7 (1999)
CAP defines a second signalling pathway required for insulin-stimulated glucose transport
Baumann, C A, et al (2000) Nature, 407:202-7 (2000)
Flotillin-1-enriched lipid raft domains accumulate on maturing phagosomes
Dermine, J F, et al (2001) J Biol Chem, 276:18507-12 (2001)
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Species Reactivity Key Applications Host Format Antibody Type
H, M WB Rabbit Purified Monoclonal Antibody
Description:
Anti-Flotillin-1 Antibody, rabbit monoclonal, clone EP446Y
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Mouse Flotillin-1.
Immunogen:
A synthetic peptide corresponding to residues in human Flotillin-1.
Clone:
EP446Y
Background Information:
Flotillin-1, also known as Reggie-2, belongs to a larger family of proteins that share an evolutionarily conserved stomatin/prohibitin/flotillin/HflK/C (SPFH) domain (1). Flotillin-1 is a lipid raft-associated protein that has been implicated in various cellular processes. Flotillin was first found in cells that lack morphologically definable caveolae, as well as in non-caveolar membranes (2) and was subsequently identified in low density detergent-insoluble complexes of endothelial cells, where they were termed flotillins (3). As well as its postulated role in neuronal regeneration, flotilllin-1 has been implicated in insulin signaling to trigger glucose transporter redistribution in adipocytes (4) Flotillin also associates with phagosomes in macrophages (5).
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human, Mouse (positive control: K562 cell lysate)
Application Notes:
WB: 1:500
Purification Method:
Protein A purfied
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
O75955
Entrez Gene Number:
NM_005803.2
Gene Symbol:
FLOT1
Flotillin-1.
Alternate Names:
Reggie-2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined.
UniProt Summary:
FUNCTION: SwissProt: O75955 # May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
SIZE: 427 amino acids; 47355 Da
SUBUNIT: Hetero-oligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2 (By similari |