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Anti-FAK Antibody, rabbit monoclonal, clone EP695Y
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REFERENCES
pp125FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions.
Schaller, M D, et al. (1992) Proc. Natl. Acad. Sci. U.S.A., 89: 5192-6 (1992)
Focal adhesion kinase (pp125FAK) expression, activation and association with paxillin and p50CSK in human metastatic prostate carcinoma
Tremblay, L, et al (1996) Int J Cancer, 68:164-71 (1996)
Overexpression of the focal adhesion kinase (p125FAK) in invasive human tumors
Owens, L V, et al (1995) Cancer Res, 55:2752-5 (1995)
Bombesin and platelet-derived growth factor induce association of endogenous focal adhesion kinase with Src in intact Swiss 3T3 cells
Salazar, E P and Rozengurt, E (1999) J Biol Chem, 274:28371-8 (1999)
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R IP, WB, ICC, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-FAK Antibody, rabbit monoclonal, clone EP695Y
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Mouse, Rat FAK.
Immunogen:
A synthetic peptide corresponding to residues in human FAK.
Clone:
EP695Y
Background Information:
Focal adhesion kinase (FAK) is a non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis (1). FAK is composed of a central catalytic domain flanked by large N- and C-terminal regions. FAK is activated by phosphorylation at tyrosine 397 in response to integrin clustering which can be induced by cell adhesion or antibody cross-linking or via G-protein-coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid (2-3). FAK expression is upregulated in various tumors (4). Increased FAK expression has been correlated with the enhanced motility and invasiveness of human tumor cells, as well as with promoting increased cell proliferation (5).
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human, Mouse, Rat (positive control: Hela cell lysate)
Application Notes:
WB: 1:1,000
IHC: 1:50-100 (paraffin-embedded human hepatocellular carcinoma)
ICC: 1:250-500 (Hela cells)
IP: 1:50
Purification Method:
Protein A purfied
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
Q05397
Entrez Gene Number:
NM_005607.3
NM_153831.2
Gene Symbol:
PTK2
pp125FAK
FAK
FADK
FAK1
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Alternate Names:
Focal adhesion kinase
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
Entrez Gene Summary:
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases |