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Anti-Caldesmon Antibody, rabbit monoclonal, clone E89
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REFERENCES
Cloning and expression of a smooth muscle caldesmon
Bryan, J, et al (1989) J Biol Chem, 264:13873-9 (1989)
Cloning of cDNAs encoding human caldesmons
Humphrey, M B, et al (1992) Gene, 112:197-204 (1992)
Caldesmon: fragments, sequence, and domain mapping
Bryan, J (1990) Ann N Y Acad Sci, 599:100-10 (1990)
The inhibitory complex of smooth muscle caldesmon with actin and tropomyosin involves three interacting segments of the C-terminal domain 4
Fraser, I D, et al (1997) Biochemistry, 36:5483-92 (1997)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R FC, IP, WB, ICC, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-Caldesmon Antibody, rabbit monoclonal, clone E89
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Mouse, Rat Caldesmon
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Ser789 of human Caldesmon.
Clone:
E89
Background Information:
Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human, Mouse, Rat (positive control: NIH/3T3 cell lysate)
Application Notes:
WB: 1:10,000-20,000
IHC: 1:250 (Paraffin-embedded human uterus)
ICC: 1:250 (Hela cells)
Flow Cytometry: 1:20
IP: 1:80
Purification Method:
Protein A purfied
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
Q05682
Entrez Gene Number:
NM_004342.5
NM_033138.2
NM_033139.2
NM_033140.2
NM_033157.2
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Gene Symbol:
CALD1
CDM
CAD
L-CAD
MGC21352
Caldesmon
NAG22
H-CAD
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
Immunoprecipitation
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
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Entrez Gene Summary:
This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle c |