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Anti-Calponin-1 (CT) Antibody, rabbit monoclonal, clone EP798Y
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REFERENCES
Calponin binds G-actin and F-actin with similar affinity
Ferjani, Imen, et al (2006) FEBS Lett, 580:4801-6 (2006)
Mammalian calponin. Identification and expression of genetic variants
Strasser, P, et al (1993) FEBS Lett, 330:13-8 (1993)
The myoepithelial immunophenotype in 135 benign and malignant salivary gland tumors other than pleomorphic adenoma
Prasad, A R, et al (1999) Arch Pathol Lab Med, 123:801-6 (1999)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, ICC, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-Calponin-1 (CT) Antibody, rabbit monoclonal, clone EP798Y
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Mouse, Rat Calponin-1 (C-Term).
Immunogen:
A synthetic peptide corresponding to residues near the C-term of human Calponin.
Clone:
EP798Y
Background Information:
Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein thought to be involved in regulation of actomyosin as well as the regulation or modulation of contraction (1). Calponin binds filamentous actin (F-actin) through two distinct sites ABS1 and ABS2, with an affinity in the low micromolar range (2) Immunoreactivity for calponin, along with alpha-smooth muscle actin and smooth muscle myosin heavy chains, confirms the known neoplastic myoepithelial component of adenoid cystic carcinomas and epithelial-myoepithelial carcinomas. The consistently positive staining pattern in adenoid cystic carcinomas may be diagnostically useful in discriminating histologically similar but consistently negative polymorphous low-grade adenocarcinomas (3).
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human, Mouse, Rat (positive control: human bladder lysate)
Application Notes:
WB: 1:20,000
IHC: 1:100 - 250 (Paraffin-embedded human smooth muscle)
ICC: 1:100 - 250 (Hela cells)
Purification Method:
Protein A purfied
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
P51911
Entrez Gene Number:
NM_001299.4
Gene Symbol:
CNN1
SMCC
Sm-Calp
Calponin-1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: SwissProt: P51911 # Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).
SIZE: 297 amino acids; 33170 Da
SUBUNIT: Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1 (By similarity).
TISSUE SPECIFICITY: Smooth muscle, and tissues containing significant amounts of smooth muscle.
SIMILARIT |