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Anti-Cytokeratin 8 Antibody, rabbit monoclonal, clone E432
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REFERENCES
Keratins and the skin.
Fuchs, E (1995) Annu. Rev. Cell Dev. Biol., 11: 123-53 (1995)
Cloning of the human keratin 18 gene and its expression in nonepithelial mouse cells
Kulesh, D A and Oshima, R G (1988) Mol Cell Biol, 8:1540-50 (1988)
Identification and mutational analysis of the glycosylation sites of human keratin 18
Ku, N O and Omary, M B (1995) J Biol Chem, 270:11820-7 (1995)
Cytokeratin expression, fibrillar organization, and subtle function in liver cells
Marceau, N and Loranger, ABiochem Cell Biol, 73:619-25 ()
Species Reactivity Key Applications Host Format Antibody Type
H, M FC, WB, ICC, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-Cytokeratin 8 Antibody, rabbit monoclonal, clone E432
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Mouse Cytokeratin 8.
Immunogen:
A synthetic peptide corresponding to residues surrounding Ser73 of human Cytokeratin 8.
Clone:
E432
Background Information:
Keratin polypeptides 8 and 18 (K8/18) are intermediate filament phosphoglycoproteins that are expressed preferentially in glandular epithelia. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells (1-3). Together with actin microfilaments and microtubules, keratin filaments make up the cytoskeletons of vertebrate epithelial cells, forming alpha-helical coiled-coil dimers which associate laterally and end-to-end to form 10-nm diameter filaments (4).
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human, Mouse (positive control: A431 cell lysate)
Application Notes:
WB: 1:500
IHC: 1:250-1:500 (Paraffin-embedded stomach carcinoma)
ICC: 1:50
Flow Cytometry: 1:20
Purification Method:
Protein A purfied
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
P05787
Entrez Gene Number:
NM_002273.2
Gene Symbol:
KRT8
Cytokeratin-8
Keratin-8
K2C8
CYK8
CK-8
KO
K8
CK8
CARD2
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: SwissProt: P05787 # Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
SIZE: 483 amino acids; 53704 Da
SUBUNIT: Heterotetramer of two type I and two type II keratins. keratin-8 associates with keratin-18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed |