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Anti-Cytokeratin 5 Antibody
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REFERENCES
Isolation, sequence, and expression of a human keratin K5 gene: transcriptional regulation of keratins and insights into pairwise control.
Lersch, R, et al. (1989) Mol. Cell. Biol., 9: 3685-97 (1989)
Expression of cytokeratin 5 and calretinin in clear-cell renal cell carcinoma
Cid Mouteira, P, et al (2006) Actas urologicas españolas , 30:367-71 (2006)
Species Reactivity Key Applications Host Format Antibody Type
H WB, ICC, IH(P) Rabbit Purified Polyclonal Antibody
Description:
Anti-Cytokeratin 5 Antibody
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human Cytokeratin 5.
Immunogen:
A synthetic peptide corresponding to residues near the N-terminus of human CK-5.
Background Information:
Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells (1-3). Together with actin microfilaments and microtubules, keratin filaments make up the cytoskeletons of vertebrate epithelial cells, forming alpha-helical coiled-coil dimers which associate laterally and end-to-end to form 10-nm diameter filaments (4).
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Species Reactivity:
Human
Species Reactivity Note:
Human (positive control: A431 cell lysate)
Application Notes:
WB: 1:1000
IHC: 1:50 - 100 (Paraffin-embedded human transitional carcinoma of bladder)
ICC: 1:50 - 100
Purification Method:
Protein A purfied
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.18 M NaCl, 40% Glycerol, 0.25% sodium azide, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
P13647
Entrez Gene Number:
NM_000424.3
Gene Symbol:
KRT5
DDD
Keratin-5
KRT5A
CK-5
CK5
K5
EBS2
Cytokeratin-5
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
Entrez Gene Summary:
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
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UniProt Summary:
SIZE: 590 amino acids; 62378 Da
SUBUNIT: Heterotetramer of two type I and two type II keratins. Keratin-5 associates with keratin-14. Interacts with TCHP.
DISEASE: SwissProt: P13647 # Defects in KRT5 are a cause of epidermolysis bullosa simplex (EBS) [MIM:131760, 131800, 131900, 131960]. Epidermolysis bullosa (EB) is a mendelian disorder that can be inherited in an autosomal dominant or recessive form and is characterized by blistering or erosion of the skin and mu |