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Anti-Cytokeratin 18 Antibody, rabbit monoclonal, clone E431-1
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REFERENCES
Keratins and the skin.
Fuchs, E (1995) Annu. Rev. Cell Dev. Biol., 11: 123-53 (1995)
Cloning of the human keratin 18 gene and its expression in nonepithelial mouse cells
Kulesh, D A and Oshima, R G (1988) Mol Cell Biol, 8:1540-50 (1988)
Identification and mutational analysis of the glycosylation sites of human keratin 18
Ku, N O and Omary, M B (1995) J Biol Chem, 270:11820-7 (1995)
Cytokeratin expression, fibrillar organization, and subtle function in liver cells
Marceau, N and Loranger, ABiochem Cell Biol, 73:619-25 ()
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, ICC, IH(P), FC Rabbit Purified Monoclonal Antibody
Description:
Anti-Cytokeratin 18 Antibody, rabbit monoclonal, clone E431-1
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Mouse, Rat Cytokeratin 18.
Immunogen:
A synthetic peptide corresponding to residues of human Cytokeratin 18.
Clone:
E431-1
Background Information:
Keratin polypeptides 8 and 18 (K8/18) are intermediate filament phosphoglycoproteins that are expressed preferentially in glandular epithelia. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells (1-3). Together with actin microfilaments and microtubules, keratin filaments make up the cytoskeletons of vertebrate epithelial cells, forming alpha-helical coiled-coil dimers which associate laterally and end-to-end to form 10-nm diameter filaments (4).
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Species Reactivity:
Human
Mouse
Rat
Application Notes:
Western Blot: 1:10,000
Immunohistochemistry: 1:100-1:250 (Paraffin-embedded colon carcinoma)
Immunocytochemistry: 1:100
Flow Cytometry: 1:20
Optimal working dilutions must be determined by the end user.
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Control:
Positive control: A431 cell lysate.
Purification Method:
Protein A purfied
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
P05783
Entrez Gene Number:
NM_000224.2
NM_199187.1
Gene Symbol:
KRT18
K18
CYK18
Keratin-18
Cytokeratin-18
CK-18
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
Flow Cytometry
Entrez Gene Summary:
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
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UniProt Summary:
FUNCTION: SwissProt: P05783 # Involved in the uptake of thrombin-anti |