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Anti-Paxillin (NT) Antibody, rabbit monoclonal, clone Y113
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REFERENCES
Tyrosine phosphorylation of paxillin and pp125FAK accompanies cell adhesion to extracellular matrix: a role in cytoskeletal assembly.
Burridge, K, et al. (1992) J. Cell Biol., 119: 893-903 (1992)
Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL
Salgia, R, et al (1995) J Biol Chem, 270:5039-47 (1995)
Characterization of tyrosine phosphorylation of paxillin in vitro by focal adhesion kinase
Bellis, S L, et al (1995) J Biol Chem, 270:17437-41 (1995)
Involvement of phosphorylation of Tyr-31 and Tyr-118 of paxillin in MM1 cancer cell migration
Iwasaki, Teruo, et al (2002) Int J Cancer, 97:330-5 (2002)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R IP, WB, ICC, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-Paxillin (NT) Antibody, rabbit monoclonal, clone Y113
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Mouse, Rat Paxillin (N-Term).
Immunogen:
A synthetic peptide corresponding to residues in N-terminus of human Paxillin. This antibody detects alpha, beta and gamma isoforms of Paxillin.
Clone:
Y113
Background Information:
Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Positive control: Hela cell lysate
Application Notes:
WB: 1:5,000-10,000
IHC: 1:100 (Paraffin-embedded breast carcinoma)
ICC: 1:250 (Hela cells)
IP: 1:200
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
P49023
Entrez Gene Number:
NM_002859.1
Gene Symbol:
PXN
Paxillin.
paxillin
FLJ16691
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: SwissProt: P49023 # Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
SIZE: 591 amino acids; 64533 Da
SUBUNIT: Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1 (By similarity). Binds |