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Anti-PAK1 (NT) Antibody, rabbit monoclonal, clone EP795Y
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REFERENCES
p21-activated kinases: three more join the Pak.
Jaffer, Zahara M and Chernoff, Jonathan (2002) Int. J. Biochem. Cell Biol., 34: 713-7 (2002)
Inhibition of myosin light chain kinase by p21-activated kinase
Sanders, L C, et al (1999) Science, 283:2083-5 (1999)
p21-activated protein kinase: a crucial component of morphological signaling?
Daniels, R H and Bokoch, G M (1999) Trends Biochem Sci, 24:350-5 (1999)
A brain serine/threonine protein kinase activated by Cdc42 and Rac1
Manser, E, et al (1994) Nature, 367:40-6 (1994)
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R FC, IP, WB, ICC, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-PAK1 (NT) Antibody, rabbit monoclonal, clone EP795Y
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Mouse, Rat PAK1 (N-Term).
Immunogen:
A synthetic peptide corresponding to residues in the N-terminus of human PAK1 protein.
Clone:
EP795Y
Background Information:
p21-activated kinase 1 (PAK1) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). PAK1 participates in regulating the actin cytoskeleton, focal adhesion contacts, cell motility, apoptosis and transcription. It also promotes the disassembly of stress fibers (4-5). PAK1 also binds PIX (PAK-binding protein), COOL and adapter protein Nck (5).
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Positive controls: Hela, A431, Jurkat, K562, MCF7, Daudi, NIH/3T3, PC12.
Application Notes:
WB: 1:500
IHC: 1:100-250 (Paraffin-embedded human breast carcinoma)
ICC: 1:50-100 (Hela cells)
Flow Cytometry: 1:10
IP: 1:50
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
Q13153
Entrez Gene Number:
NM_002576.3
Gene Symbol:
PAK1
p65-PAK
MGC130001
Alpha-PAK
MGC130000
PAK-1
PAKalpha
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Alternate Names:
p21-activated kinase 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
Immunoprecipitation
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
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Entrez Gene Summary:
PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternative transcripts of this gene have been found, but their full-length natures hav |