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Anti-Apaf-1 Antibody, rabbit monoclonal, clone E38
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REFERENCES
Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3.
Zou, H, et al. (1997) Cell, 90: 405-13 (1997)
Cytochrome c and dATP-mediated oligomerization of Apaf-1 is a prerequisite for procaspase-9 activation
Saleh, A, et al (1999) J Biol Chem, 274:17941-5 (1999)
Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis
Hu, Y, et al (1999) EMBO J, 18:3586-95 (1999)
Oligomerization and activation of caspase-9, induced by Apaf-1 CARD
Shiozaki, Eric N, et al (2002) Proc Natl Acad Sci USA, 99:4197-202 (2002)
Species Reactivity Key Applications Host Format Antibody Type
H, M WB, ICC, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-Apaf-1 Antibody, rabbit monoclonal, clone E38
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Mouse Apaf-1.
Immunogen:
A synthetic peptide corresponding to residues near N-terminus of human Apaf-1.
Clone:
E38
Background Information:
Apaf-1, or apoptotic protease activating factor 1, is a human protein homolog of the C. elagans apoptotic regulatory protein CED-4 that activates caspase-9 during apoptosis (1). Cytosolic Apaf-1 forms an oligomeric complex with caspase-9 in the presence of cytochrome c and dATP, which leads to caspase-9 activation and subsequent activation of caspase-3 (2, 3). Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains, consequently enhancing the catalytic activity of caspase-9 (4), leading to activation of the caspase signaling cascade and apoptosis.
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human, Mouse (positive control: Hela cell lysate)
Application Notes:
WB: 1:500
IHC: 1:50-1:100 (Paraffin-embedded stomach adenocarcinoma)
ICC: 1:50-1:100
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
O14727
Entrez Gene Number:
NM_001160.2
NM_013229.2
NM_181861.1
NM_181868.1
NM_181869.1
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Gene Symbol:
APAF1
DKFZp781B1145
Apaf-1
KIAA0413
CED4
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Alternate Names:
apoptotic protease activating factor 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
Entrez Gene Summary:
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encod |