|
Anti-Smac/DIABLO Antibody, rabbit monoclonal, clone Y12
Close
REFERENCES
Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition.
Du, C, et al. (2000) Cell, 102: 33-42 (2000)
Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins
Verhagen, A M, et al (2000) Cell, 102:43-53 (2000)
Structural basis for binding of Smac/DIABLO to the XIAP BIR3 domain.
Liu, Z, et al. (2000) Nature, 408: 1004-8 (2000)
Structural basis of IAP recognition by Smac/DIABLO.
Wu, G, et al. (2000) Nature, 408: 1008-12 (2000)
Species Reactivity Key Applications Host Format Antibody Type
H, R FC, IP, WB, ICC, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-Smac/DIABLO Antibody, rabbit monoclonal, clone Y12
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Rat Smac/Diablo.
Immunogen:
A synthetic peptide corresponding to C-terminal residues human Smac/DIABLO.
Clone:
Y12
Background Information:
Smac/DIABLO (Second Mitochondria-derived Activator of Caspases/Direct IAP Binding Protein with Low PI) is localized to the mitochondria and involved in activating programmed cell death, or apoptosis (1). In response to apoptotic stimuli, Smac/DIABLO is released from the mitochondria and binds anti-apoptotic IAP proteins, supressing their inhibitory activity and promoting caspase activation (1,2). This interaction involves primarily the amino-terminal residues of Smac/DIABLO with the BIR3 region of the IAP protein (3,4).
View All »
Species Reactivity:
Human
Rat
Species Reactivity Note:
Human, Rat (positive control: Jurkat cell lysate)
Application Notes:
WB: 1:1,000
IHC: 1:10 (Paraffin-embedded human skin cancer tissue)
ICC: 1:10
Flow Cytometry: 1:10-50
IP: 1:10-80
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
Q9NR28
Entrez Gene Number:
NM_019887.3
NM_138929.2
NM_138930.2
Gene Symbol:
DIABLO
SMAC
DIABLO-S
FLJ25049
0610041G12Rik
diablo
FLJ10537
SMAC3
View All »
Alternate Names:
Second Mitochondria-derived Activator of Caspases/Direct IAP Binding Protein with Low PI
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Flow Cytometry
Immunoprecipitation
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
View All »
Entrez Gene Summary:
This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Several alternatively spliced transcript variants that encode distinct isoforms have been described for this gene but the validity of some transcripts, and their predicted ORFs, has not been determined conclusively.
View All &ra |