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Anti-RasGAP Antibody, rabbit monoclonal, clone EP536Y
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REFERENCES
Surviving the kiss of death.
Yang, Jiang-Yan, et al. (2004) Biochem. Pharmacol., 68: 1027-31 (2004)
Antiapoptotic signaling generated by caspase-induced cleavage of RasGAP
Yang, J Y and Widmann, C (2001) Mol Cell Biol, 21:5346-58 (2001)
Partial cleavage of RasGAP by caspases is required for cell survival in mild stress conditions
Yang, Jiang-Yan, et al (2004) Mol Cell Biol, 24:10425-36 (2004)
Impaired Akt activity down-modulation, caspase-3 activation, and apoptosis in cells expressing a caspase-resistant mutant of RasGAP at position 157
Yang, Jiang-Yan, et al (2005) Mol Biol Cell, 16:3511-20 (2005)
Species Reactivity Key Applications Host Format Antibody Type
H, R ICC, IH(P), WB Rabbit Purified Monoclonal Antibody
Description:
Anti-RasGAP Antibody, rabbit monoclonal, clone EP536Y
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Rat RasGap.
Immunogen:
A synthetic peptide corresponding to residues near the SH3 domain of human RasGAP.
Clone:
EP536Y
Background Information:
RasGAP, a regulator of Ras and Rho, stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. RasGAP is a Caspase 3 substrate (1) and acts as both pro- and anti-apoptotic protein depending on the extent of its cleavage by caspases (2). At low levels of caspase activity, RasGAP is cleaved at position 455, generating an N-terminal fragment (fragment N) and a C-terminal fragment (fragment C). Fragment C alone can induce apoptosis, but this response is completely inhibited by fragment N (3). Fragment N appears to be a general blocker of apoptosis downstream of caspase activation because it inhibits caspase 9-induced cell death. At higher caspase activity, fragment N is further processed and Cleavage of fragment N abrogates its protective functions, and hence the second cleavage of RasGAP promotes apoptosis (4).
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Species Reactivity:
Human
Rat
Species Reactivity Note:
Human, Rat (positive control: Rat brain lysate)
Application Notes:
WB: 1:2000
IHC: 1:50 (Paraffin-embedded human ovary carcinoma)
ICC: 1:250 - 500 (Hela cells)
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
P20936
Entrez Gene Number:
NM_002890.1
NM_022650.1
Gene Symbol:
RASA1
CMAVM
CM-AVM
RASA
PKWS
GAP
p120GAP
RASGAP
RasGAP
DKFZp434N071
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunocytochemistry
Immunohistochemistry (Paraffin)
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP |