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Anti-PARP-1 (Cleaved p25) Antibody, rabbit monoclonal
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REFERENCES
Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase.
Tewari, M, et al. (1995) Cell, 81: 801-9 (1995)
The zinc fingers of human poly(ADP-ribose) polymerase are differentially required for the recognition of DNA breaks and nicks and the consequent enzyme activation. Other structures recognize intact DNA.
Ikejima, M, et al. (1990) J. Biol. Chem., 265: 21907-13 (1990)
Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis
Kaufmann, S H, et al (1993) Cancer Res, 53:3976-85 (1993)
Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE
Lazebnik, Y A, et al (1994) Nature, 371:346-7 (1994)
Species Reactivity Key Applications Host Format Antibody Type
H, M WB, FC, IP, ICC, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-PARP-1 (Cleaved p25) Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human, Mouse PARP-1 (Cleaved p25).
Immunogen:
A synthetic peptide corresponding to residues before the cleavage site of human PARP-1. The antibody only recognize p25 cleaved-form of PARP-1.
Clone:
E51
Background Information:
Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis, PARP is cleaved in vitro by many caspases, and in vivo by Caspase-3 (2,3). Existing as a 116 kDa nuclear protein, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29 kDa (C-terminal catalytic domain) and 85 kDa (N-terminal DNA-binding domain) (2,4).
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human, Mouse (positive control: Jurkat cell lysate)
Application Notes:
WB: 1:10,000-50,000
IHC: 1:100 (Paraffin-embedded human breast carcinoma tissue)
ICC: 1:250 (Hela cell)
Flow Cytometry: 1:50
IP: 1:50
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
P09874
Entrez Gene Number:
NM_001618.2
Gene Symbol:
PARP1
NAD
Poly
PPOL
poly(ADP-ribosyl)transferase
PARP-1
PARP
pADPRT-1
ADPRT1
ADPRT
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Alternate Names:
poly (ADP-ribose) polymerase-1
ARTD1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Flow Cytometry
Immunoprecipitation
Immunocytochemistry
Immunohistochemistry (Paraffin)
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Entrez Gene Summary:
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tum |