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Anti-PARP-1 (p116/p25) Antibody, rabbit monoclonal
Species Reactivity Key Applications Host Format Antibody Type
H IP, WB Rabbit Purified Monoclonal Antibody
Description:
Anti-PARP-1 (p116/p25) Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human PARP-1 (p116/p25).
Immunogen:
A synthetic peptide corresponding to residues before the cleavage site of human PARP-1. The antibody only recognizes full-length form (p116) of PARP-1.
Clone:
Y17
Background Information:
Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis, PARP is cleaved in vitro by many caspases, and in vivo by Caspase-3 (2,3). Existing as a 116 kDa nuclear protein, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29 kDa (C-terminal catalytic domain) and 85 kDa (N-terminal DNA-binding domain) (2,4).
Species Reactivity:
Human
Species Reactivity Note:
Human (positive control: Jurkat cell lysate)
Application Notes:
WB: 1:1000 IP: 1:80
Presentation:
Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Store at -20 °C. Stable for 12 months from date of receipt.
UniProt Number:
P09874
Entrez Gene Number:
NM_001618.2
Gene Symbol:
PARP1
NAD
Poly
PPOL
poly(ADP-ribosyl)transferase
PARP-1
PARP
pADPRT-1
ADPRT1
ADPRT
Alternate Names:
poly (ADP-ribose) polymerase-1
ARTD1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
UniProt Summary:
FUNCTION: SwissProt: P09874 # Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.
SIZE: 1014 amino acids; 113084 Da
SUBUNIT: Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with ZNF423 (By similarity).
SUBCELLULAR LOCATION: Nucleus.
PTM: Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR. & Poly-ADP-ribosylated by PARP2.
SIMILARITY: SwissProt: P09874 ## Contains 1 BRCT domain. & |