|
Anti-SREBP-2 Antibody, clone 2545
Close
REFERENCES
The hypocholesterolemic agent LY295427 reverses suppression of sterol regulatory element-binding protein processing mediated by oxysterols
Janowski, B A, et al (2001) J Biol Chem, 276:45408-16 (2001)
Species Reactivity Key Applications Host Format Antibody Type
H ELISA, WB Mouse Ascites Monoclonal Antibody
Description:
Anti-SREBP-2 Antibody, clone 2545
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Reacts with both the precursor and mature forms of SREBP-2,
Molecular Weight:
124 kDa
Immunogen:
Recombinant GST-tagged human SREBP-2 protein encompassing amino acids 48-403.
Clone:
Clone 2545
Isotype:
IgG1
Species Reactivity:
Human
Quality Assurance:
Routinely evaluated by immunoblot.
Presentation:
200 μL of mouse ascites of monoclonal IgG1 with 0.1% sodium azide.
Storage Conditions:
Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/ thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
View All »
UniProt Number:
Q12772
Entrez Gene Number:
NM_004599.2
Gene Symbol:
SREBF2
OTTHUMP00000028740
SREBP-2
SREBP2
Alternate Names:
SREBP2, sterol regulatory element binding transcription factor 2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
ELISA
Western Blotting
Entrez Gene Summary:
This gene encodes a ubiquitously expressed transcription factor that controls cholesterol homeostasis by stimulating transcription of sterol-regulated genes. The encoded protein contains a basic helix-loop-helix-leucine zipper (bHLH-Zip) domain.
UniProt Summary:
FUNCTION: SwissProt: Q12772 # Transcriptional activator that binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.
SIZE: 1141 amino acids; 123688 Da
SUBUNIT: Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein.
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
TISSUE SPECIFICITY: Ubiquitously expressed in adult and fetal tissues.
PTM: Under sterol-depleted conditions, SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage occurs within the luminal loop and is directly regulated by sterols. The second cleavage by site-2 protease occurs within the first transmembrane stretch and liberates the soluble transcription factor. Cleavage by the cysteine proteases, caspase- 3 and caspase-7, is induced during apoptosis, independent of sterol levels.
SIMILARITY: SwissProt: Q12772 ## Contains 1 basic helix-loop-helix (bHLH) domain.
|