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Anti-phospho-STAT1 (Ser 727) Antibody, clone 12C5
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REFERENCES
VEGF receptors on chronic lymphocytic leukemia (CLL) B cells interact with STAT 1 and 3: implication for apoptosis resistance.
Lee, Y K, et al. (2005) Leukemia, 19: 513-23 (2005)
Differential effects of lipoprotein lipase on tumor necrosis factor-alpha and interferon-gamma-mediated gene expression in human endothelial cells
Kota, Rama S, et al (2005) J Biol Chem, 280:31076-84 (2005)
Roles of phosphatidylinositol 3-kinase in interferon-gamma-dependent phosphorylation of STAT1 on serine 727 and activation of gene expression
Nguyen, H, et al (2001) J Biol Chem, 276:33361-8 (2001)
Prostacyclin receptor induces STAT1 and STAT3 phosphorylations in human erythroleukemia cells: a mechanism requiring PTX-insensitive G proteins, ERK and JNK
Lo, Rico K H, et al (2006) Cell Signal, 18:307-17 (2006)
Species Reactivity Key Applications Host Format Antibody Type
H ELISA, WB Mouse Purified Monoclonal Antibody
Description:
Anti-phospho-STAT1 (Ser 727) Antibody, clone 12C5
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes phosphorylated domain of STAT1 on Serine727
Molecular Weight:
92 kDa
Immunogen:
KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Serine727 on phosphorylated STAT1
Modifications:
Phosphorylation
Clone:
12C5
Isotype:
IgG1
Species Reactivity:
Human
Quality Assurance:
Routinely evaluated by immunoblot.
Purification Method:
Subsequent thiophilic adsorption and size exclusion chromatography
Presentation:
In 1 mL of PBS containing 0.09% sodium azide, PEG, sucrose, and 50% glycerol.
Storage Conditions:
2 years at -20°C from date of shipment. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P42224
Entrez Gene Number:
NM_007315.2
NM_139266.1
Gene Symbol:
STAT1
ISGF-3
STAT91
DKFZp686B04100
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
ELISA
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.
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UniProt Summary:
FUNCTION: SwissProt: P42224 # Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to |