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Anti-Daxx Antibody, rabbit monoclonal
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REFERENCES
Promyelocytic leukemia protein (PML) and Daxx participate in a novel nuclear pathway for apoptosis.
Zhong, S, et al. (2000) J. Exp. Med., 191: 631-40 (2000)
Daxx, a novel Fas-binding protein that activates JNK and apoptosis
Yang, X, et al (1997) Cell, 89:1067-76 (1997)
Species Reactivity Key Applications Host Format Antibody Type
H FC, IF, WB, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-Daxx Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes a region composed of residues of human Daxx.
Molecular Weight:
110 kDa
Immunogen:
KLH-conjugated synthetic peptide composed of residues of human Daxx.
Isotype:
IgG
Species Reactivity:
Human
Quality Assurance:
Routinely evaluated by immunoblot.
Presentation:
In 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Storage Conditions:
2 year at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
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UniProt Number:
Q9UER7
Entrez Gene Number:
NM_001350.3
Gene Symbol:
DAXX
MGC126246
Daxx
BING2
hDaxx
MGC126245
EAP1
DAP6
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
Immunofluorescence
Western Blotting
Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: SwissProt: Q9UER7 # Proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. Down-regulates basal and activated transcription. Seems to act as a transcriptional co- repressor and inhibits PAX3 and ETS1 through direct protein- protein interaction. Modulates PAX5 activity. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively.
SIZE: 740 amino acids; 81373 Da
SUBUNIT: Homomultimer. Binds to the TNFRSF6 death domain via its C-terminus and to PAX5. Binds to SLC2A4/GLUT4, MAP3K5, TGFBR2, phosphorylated dimeric HSPB1/HSP27, CENPC1, ETS1, sumoylated PML, UBE2I and MCRS1. Is part of a complex containing PAX5 and CREBBP. Interacts with HIPK2 and HIPK3 via its N-terminus. Interacts with HIPK1, which induces translocation from PML/POD/ND10 nuclear bodies to chromatin and enhances association with HDAC1 (By similarity). The non-phosphorylated |