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Anti-HSP27 Phospho (Ser78) Antibody, rabbit monoclonal
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REFERENCES
The diterpenoid alkaloid noroxoaconitine is a Mapkap kinase 5 (MK5/PRAK) inhibitor.
Sergiy Kostenko,Mahmud Tareq Hassan Khan,Ingebrigt Sylte,Ugo Moens (2011) Cellular and molecular life sciences : CMLS.68
The 28-kDa protein whose phosphorylation is induced by protein kinase C activators in MCF-7 cells belongs to the family of low molecular mass heat shock proteins and is the estrogen-regulated 24-kDa protein
Faucher, C, et al (1993) J Biol Chem, 268:15168-73 (1993)
The 29-kDa proteins phosphorylated in thrombin-activated human platelets are forms of the estrogen receptor-related 27-kDa heat shock protein
Mendelsohn, M E, et al (1991) Proc Natl Acad Sci USA, 88:11212-6 (1991)
Species Reactivity Key Applications Host Format Antibody Type
H IF, IP, WB, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-HSP27 Phospho (Ser78) Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes a region composed of residues juxtaposed to Serine 78 of human HSP27.
Molecular Weight:
27 kDa
Immunogen:
KLH-conjugated synthetic phosphor-peptide composed of residues juxtaposed to Serine 78 of human HSP27.
Modifications:
Phosphorylation
Isotype:
IgG
Species Reactivity:
Human
Quality Assurance:
Routinely evaluated by immunoblot.
Presentation:
In 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Storage Conditions:
2 year at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
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UniProt Number:
P04792
Entrez Gene Number:
NM_001540.2
Gene Symbol:
HSPB1
Hs.76067
HSP27
Hsp25
HspB1
HSP28
CMT2F
HS.76067
DKFZp586P1322
HMN2B
SRP27
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunofluorescence
Immunoprecipitation
Western Blotting
Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: SwissProt: P04792 # Involved in stress resistance and actin organization.
SIZE: 205 amino acids; 22783 Da SUBUNIT: Interacts with TGFB1I1 (By similarity). Associates with alpha- and beta-tubulin and microtubules. Interacts with HSPB8 and HSPBAP1.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.
PTM: Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
DISEASE: SwissProt: P04792 # Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot- Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-M |