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Anti-Caspase-9 (Pro) Antibody, rabbit monoclonal
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REFERENCES
Reduced apoptosis and cytochrome c-mediated caspase activation in mice lacking caspase 9.
Kuida, K, et al. (1998) Cell, 94: 325-37 (1998)
ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis
Duan, H, et al (1996) J Biol Chem, 271:1621-5 (1996)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R FC, WB, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-Caspase-9 (Pro) Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes a region composed of residues at the N-terminus of human Pro-Caspase-9. This antibody only binds to the pro-form of Caspase-9.
Molecular Weight:
46 kDa
Immunogen:
KLH-conjugated synthetic peptide composed of residues at the N-terminus of human Caspase-9.
Isotype:
IgG
Species Reactivity:
Human
Mouse
Rat
Quality Assurance:
Routinely evaluated by immunoblot.
Presentation:
In 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Storage Conditions:
2 year at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
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UniProt Number:
P55211
Entrez Gene Number:
NM_001229.2
NM_032996.1
Gene Symbol:
CASP9
CASP-9
ICE-LAP6
MCH6
APAF3
CASPASE-9c
OTTHUMP00000044594
APAF-3
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
Western Blotting
Immunohistochemistry (Paraffin)
Entrez Gene Summary:
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms.
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UniProt Summary:
FUNCTION: SwissProt: P55211 # Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
SIZE: 416 amino acids; 46281 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C and ATP. Interacts with the inhibitors BIRC2, BIRC4, BIRC5 and BIRC7.
TISSUE SPECIFICITY: Ubiquitous, wi |