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Anti-Caspase-3 (Pro) Antibody, rabbit monoclonal
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REFERENCES
Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase.
Tewari, M, et al. (1995) Cell, 81: 801-9 (1995)
CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme.
Fernandes-Alnemri, T, et al. (1994) J. Biol. Chem., 269: 30761-4 (1994)
Species Reactivity Key Applications Host Format Antibody Type
H, M FC, IF, IP, WB, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-Caspase-3 (Pro) Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes a region composed of residues at the N-terminus of human Pro-Caspase-3. This antibody will only detect the pro-form of Caspase-3
Molecular Weight:
35 kDa
Immunogen:
KLH-conjugated synthetic peptide composed of residues at the N-terminus of human Pro-Caspase-3.
Isotype:
IgG
Species Reactivity:
Human
Mouse
Quality Assurance:
Routinely evaluated by immunoblot.
Presentation:
In 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Storage Conditions:
2 year at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
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UniProt Number:
P42574
Entrez Gene Number:
NM_004346.3
NM_032991.2
Gene Symbol:
CASP3
apopain
CASP-3
procaspase3
CPP32B
SCA-1
CPP-32
Apopain
CPP32
Yama
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
Immunofluorescence
Immunoprecipitation
Western Blotting
Immunohistochemistry (Paraffin)
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Entrez Gene Summary:
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
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UniProt Summary:
FUNCTION: SwissProt: P42574 # Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) |