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Anti-Bax Antibody, rabbit monoclonal
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REFERENCES
Apigenin Induces Apoptosis through Mitochondrial Dysfunction in U-2 OS Human Osteosarcoma Cells and Inhibits Osteosarcoma Xenograft Tumor Growth in Vivo.
Chin-Chung Lin,Ya-Ju Chuang,Chien-Chih Yu,Jai-Sing Yang,Chi-Cheng Lu,Jo-Hua Chiang,Jing-Pin Lin,Nou-Ying Tang,An-Cheng Huang,Jing-Gung Chung (2012) Journal of agricultural and food chemistry.60
Bax interacts with the permeability transition pore to induce permeability transition and cytochrome c release in isolated mitochondria
Narita, M, et al (1998) Proc Natl Acad Sci USA, 95:14681-6 (1998)
Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death.
Oltvai, Z N, et al. (1993) Cell, 74: 609-19 (1993)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R IP, WB, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-Bax Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes a region near the dimerisation domain of human Bax.
Molecular Weight:
21 kDa
Immunogen:
Synthetic peptide corresponding to amino acid residues near the dimerisation domain of human Bax.
Isotype:
IgG
Species Reactivity:
Human
Mouse
Rat
Application Notes:
Immunoblot Analysis:
A 1:2,000 dilution of the lot detected endogenous Bax in lysate from Hela cells.
Immunoprecipitation:
A 1:100 dilution of this antibody immunoprecipitated Bax using protein G:agarose beads.
Immunohistochemistry:
1:250 of this antibody was used in Immunohistochemical analysis of Paraffin-embedded human lymph node.
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Quality Assurance:
Routinely evaluated by immunoblot.
Presentation:
In 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Storage Conditions:
2 year at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
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UniProt Number:
Q07814
Entrez Gene Number:
NM_004324.3
NM_138761.2
NM_138763.2
NM_138764.2
NM_138765.2
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Gene Symbol:
BAX
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
Immunohistochemistry (Paraffin)
Entrez Gene Summary:
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The express |