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Anti-Rap1GAP Antibody, rabbit monoclonal
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REFERENCES
Thyroid-stimulating hormone/cAMP and glycogen synthase kinase 3beta elicit opposing effects on Rap1GAP stability
Tsygankova, Oxana M, et al (2004) J Biol Chem, 279:5501-7 (2004)
Cannabinoid receptor-induced neurite outgrowth is mediated by Rap1 activation through G(alpha)o/i-triggered proteasomal degradation of Rap1GAPII
Jordan, J Dedrick, et al (2005) J Biol Chem, 280:11413-21 (2005)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R FC, IF, IP, WB, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-Rap1GAP Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes the domain N-terminal to the GoLoco motif of Rap1GAP.
Molecular Weight:
85 and 95 kDa
Immunogen:
KLH-conjugated synthetic peptide corresponding to the region N-terminus of the GoLoco motif of human Rap1GAP.
Isotype:
IgG
Species Reactivity:
Human
Mouse
Rat
Application Notes:
Suggested Dilutions:
Immunoblot Analysis: 1:50,000
Immunoprecipitation: 1:60
Flow Cytometry: 1:50
Immunohistochemistry (PE): 1:100 - 1:250
Immunfluorescence Staining: 1:100 - 1:250
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Quality Assurance:
Routinely evaluated by immunoblot.
Presentation:
100 μL of rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 0.09 M NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
Storage Conditions:
2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
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UniProt Number:
P47736
Entrez Gene Number:
NM_002885.1
Gene Symbol:
RAP1GAP
KIAA0474
RAP1GA1
Rap1GAP
rap1GAPII
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Alternate Names:
RAP1 GTPase activating protein, RAP1GA1, rap1GAPII
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
Immunofluorescence
Immunoprecipitation
Western Blotting
Immunohistochemistry (Paraffin)
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UniProt Summary:
FUNCTION: SwissProt: P47736 # GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.
SIZE: 663 amino acids; 73391 Da
SUBCELLULAR LOCATION: Golgi apparatus membrane; Peripheral membrane protein.
TISSUE SPECIFICITY: Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues.
SIMILARITY: SwissProt: P47736 ## Contains 1 GoLoco domain. & Contains 1 Rap-GAP domain.
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Brand Family:
Upstate
Product Name:
Anti-Rap1GAP Antibody, rabbit monoclonal
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µ |