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Anti-Raf-1 Antibody, rabbit monoclonal
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REFERENCES
Raf-1 serine 338 phosphorylation plays a key role in adhesion-dependent activation of extracellular signal-regulated kinase by epidermal growth factor.
Edin, Matthew L and Juliano, Rudy L (2005) Mol. Cell. Biol., 25: 4466-75 (2005)
Identification of Raf-1 S471 as a novel phosphorylation site critical for Raf-1 and B-Raf kinase activities and for MEK binding
Zhu, Jun, et al (2005) Mol Biol Cell, 16:4733-44 (2005)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R IF, IH(P), IP, WB Rabbit Purified Monoclonal Antibody
Description:
Anti-Raf-1 Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Raf-1.
Molecular Weight:
74 kDa
Immunogen:
KLH-conjugated synthetic peptide corresponding to the central region of human Raf-1.
Isotype:
IgG
Species Reactivity:
Human
Mouse
Rat
Application Notes:
Suggested Dilutions:
Immunoblot Analysis: 1:500
Immunoprecipitation: 1:100
Immunohistochemistry (PE): 1:50 - 1:100
Immunfluorescence Staining: 1:50 - 1:100
Quality Assurance:
Routinely evaluated by immunoblot.
Presentation:
100 μL of rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
Storage Conditions:
2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
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UniProt Number:
P04049
Entrez Gene Number:
NM_002880.2
Gene Symbol:
RAF1
cRaf
Raf-1
c-Raf
RAF
CRAF
C-RAF
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Alternate Names:
RAF1, CRAF, c-Raf, v-raf-1 murine leukemia viral oncogene homolog 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunofluorescence
Immunohistochemistry (Paraffin)
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. [Contributed text]
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UniProt Summary:
FUNCTION: SwissProt: P04049 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
COFACTOR: Binds 2 zinc ions per subunit.
SIZE: 648 amino aci |