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Anti-PI3 Kinase Antibody, p85α rabbit monoclonal
Species Reactivity Key Applications Host Format Antibody Type
H, M, R FC, ICC, IF, IH(P), IP, WB Rabbit Purified Monoclonal Antibody
Description:
Anti-PI3 Kinase Antibody, p85α rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes the C-terminal domain of PI3 Kinase, p85α subunit.
Molecular Weight:
85 kDa
Epitope:
C-terminus
Immunogen:
KLH-conjugated synthetic peptide corresponding to the C-terminal region of human PI3 Kinase, p85α subunit.
Isotype:
IgG
Background Information:
PI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.
Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Proven to react with human, mouse and rat.
Reactivity with other species has not been confirmed.
Application Notes:
Immunoprecipitation:
A 1:50 dilution of a previous lot of this antibody immunoprecipitated PI3K, p85α using protein G:agarose beads.
Flow Cytometry:
A 1:50 dilution of a previous lot of this antibody stained cells for intracellular staining for flow cytometry analysis.
Control:
Lysates from A431, MCF-7 or Raji cells
Quality Assurance:
Routinely evaluated by Western Blot on A431 lysate.
Western Blot Analysis:
A 1:2,000 dilution of the lot detected endogenous PI3K, p85α in lysates from A431 cells.
Purification Method:
Protein A purfied
Presentation:
Purified rabbit monoclonal IgG in buffer containing 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
UniProt Number:
P27986
Entrez Gene Number:
NM_181504.2
Gene Symbol:
PIK3R1
GRB1
PI3K
p85-ALPHA
Alternate Names:
PI3-kinase p85 subunit alpha
PtdIns-3-kinase p85-alpha
phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85
alpha)
phosphatidylinositol 3-kinase, regulatory, 1
phosphatidylinositol 3-kinase-associated p-85 alpha
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
phosphoinositide-3-kinase, regulatory subunit 1 (p85 alpha)
phosphoinositide-3-kinase, regulatory subunit, polypeptide 1 (p85
alpha).
Usage Statement:
Unless otherwise stated in our catalog or other company documentation acco |