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Anti-PI3 Kinase Antibody, p110γ, rabbit monoclonal
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REFERENCES
Identification and characterization of the autophosphorylation sites of phosphoinositide 3-kinase isoforms beta and gamma
Czupalla, Cornelia, et al (2003) J Biol Chem, 278:11536-45 (2003)
Oncogenic transformation induced by the p110beta, -gamma, and -delta isoforms of class I phosphoinositide 3-kinase
Kang, Sohye, et al (2006) Proc Natl Acad Sci USA, 103:1289-94 (2006)
Species Reactivity Key Applications Host Format Antibody Type
H FC, IP, WB Rabbit Purified Monoclonal Antibody
Description:
Anti-PI3 Kinase Antibody, p110γ, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes the N-terminus human PI3 Kinase, p110γ.
Molecular Weight:
110 kDa
Epitope:
N-terminus
Immunogen:
KLH-conjugated synthetic peptide corresponding to the N-terminus human PI3 Kinase, p110γ
Isotype:
IgG
Background Information:
PI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.
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Species Reactivity:
Human
Species Reactivity Note:
Proven to react with human. Reactivity with other species has not been confirmed.
Application Notes:
Immunoprecipitation:
A 1:50 dilution of a previous lot of antibody immunoprecipitated PI3K, p110γ using protein G:agarose beads.
Flow Cytometry:
A 1:50 of a previous lot of this antibody stained cells for intracellular staining for flow Cytometry analysis.
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Control:
Jurkat cell lysate
Quality Assurance:
Routinely evaluated by Western Blot on jurkat cell lysate.
Western Blot Analysis:
A 1:1,000 dilution of the lot detected endogenous PI3 Kinase, p110γ in lysates from Jurkat cells.
Purification Method:
Purified
Presentation:
Purified rabbit monoclonal IgG in buffer containing 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
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UniProt Number:
P48736
Entrez Gene Number:
NM_002649.2
Gene Symbol:
PIK3CG
PI3CG
p110-gamma
PI3-kinase
PI3Kgamma
PTDINS-3-kinase
PIK3
PI3K
p120-PI3K
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Alternate Names:
PIK3CG, PI3CG, PI3K, PI3Kgamma, PIK3, 1-phosphatidylinositol 3-kinase
PI3-kinase
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