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Anti-phospho-MSK1 (S376) Antibody, rabbit monoclonal
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REFERENCES
Cell type-specific targeting dissociates the therapeutic from the adverse effects of protein kinase inhibition in allergic skin disease.
Patcharee Ritprajak,Morisada Hayakawa,Yasuyo Sano,Kinya Otsu,Jin Mo Park (2012) Proceedings of the National Academy of Sciences of the United States of America.109
Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38, and may mediate activation of CREB
Deak, M, et al (1998) EMBO J, 17:4426-41 (1998)
Activation of the mitogen- and stress-activated kinase 1 by arsenic trioxide
Kannan-Thulasiraman, Padma, et al (2006) J Biol Chem, 281:22446-52 (2006)
Mitogen- and stress-activated protein kinase 1 is activated in lesional psoriatic epidermis and regulates the expression of pro-inflammatory cytokines
Funding, Anne T, et al (2006) J Invest Dermatol, 126:1784-91 (2006)
Species Reactivity Key Applications Host Format Antibody Type
H FC, IH(P), IP, WB Rabbit Purified Monoclonal Antibody
Description:
Anti-phospho-MSK1 (S376) Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes MSK1 when phosphorylated on Ser376.
Molecular Weight:
90kDa
Immunogen:
KLH-conjugated synthetic phospho-peptide corresponding to residues surrounding Serine 376 of human MSK1.
Modifications:
Phosphorylation
Isotype:
IgG
Species Reactivity:
Human
Application Notes:
Western Blot: 1:5000.
Immunoprecipitation: 1:30.
Immunofluorescence: 1:100-1:250.
Immunohistochemistry: 1:50-1:100 on paraffin-embedded sections of human urinary bladder carcinoma.
Optimal working dilutions must be determined by the end user.
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Control:
POSITIVE: K562 cells.
Quality Assurance:
Routinely evaluated by immunoblot analysis.
Presentation:
Rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
Storage Conditions:
2 years at -20°C from date of shipment.
UniProt Number:
O75582
Entrez Gene Number:
NM_004755.2
NM_182398.1
Gene Symbol:
RPS6KA5
RLPK
MGC1911
MSPK1
RSKL
MSK1
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Alternate Names:
Ribosomal protein S6 kinase
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: O75582 # Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of his |