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Anti-mTOR Antibody, rabbit monoclonal
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REFERENCES
Inhibition of tumor cell growth, proliferation and migration by X-387, a novel active-site inhibitor of mTOR.
Si-meng Chen,Jia-li Liu,Xiang Wang,Chris Liang,Jian Ding,Ling-hua Meng (2012) Biochemical pharmacology.83
Epigallocatechin gallate (EGCG), a major component of green tea, is a dual phosphoinositide-3-kinase/mTOR inhibitor.
Van Aller GS, Carson JD, Tang W, Peng H, Zhao L, Copeland RA, Tummino PJ, Luo L (2011) Biochem Biophys Res Commun.406:194-9. Epub 2011 Feb 15.
Safety analysis of the diaphragm in combination with lubricant or acidifying microbicide gels: effects on markers of inflammation and innate immunity in cervicovaginal fluid.
Deborah J Anderson,D'Nyce L Williams,Susan A Ballagh,Kurt Barnhart,Mitchell D Creinin,Daniel R Newman,Frederick P Bowman,Joseph A Politch,Ann C Duerr,Denise J Jamieson (2009) American journal of reproductive immunology (New York, N.Y. : 1989).61
Species Reactivity Key Applications Host Format Antibody Type
H, M, R FC, ICC, IH(P), IP, WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-mTOR Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes a region in the C-terminus domain of human mTOR.
Molecular Weight:
289 kDa
Immunogen:
KLH-conjugated synthetic peptide corresponding to the C-terminal region of human mTOR.
Background Information:
mTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. Interestingly, mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins. These two multimeric protein complexes are designated mTORC1 and mTORC2. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and GL (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. The complex is activated primarily though the PI3 Kinase/Akt pathway. Upon insulin stimulation, Akt activates mTORC1 by phosphorylating and inhibiting TSC (and possibly other yet discovered targets and/or mTOR itself). This inhibits the upstream small GTPase regulator Rheb (Ras homolog enriched in brain). This inhibits the kinase activity of the mTORC1 complex, thus disabling its ability to phosphorylate its downstream targets such as p70 S6K on Thr389 and 4E-BP1 on Thr229. The other mTOR complex, mTORC2 (mTOR Complex 2), is made up of at least mTOR, Rictor, GL, Sin1, Protor 1 and 2. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Interestingly, unlike mTORC1, the mTORC2 complex appears to not be inhibited by treatment with rapamycin and for this reason is referred to as the rapamycin-insensitive complex. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.
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Species Reactivity:
Human
Mouse
Rat
Quality Assurance:
Routinely evaluated by immunoblot.
Presentation:
100 μL of rabbit monoclonal IgG in 60% storage buffer (50 mM Tris |