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Anti-MKK6 NT Antibody, rabbit monoclonal
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REFERENCES
Regulation of p38 MAPK by MAPK kinases 3 and 6 in fibroblast-like synoviocytes.
Inoue, Tomoyuki, et al. (2005) J. Immunol., 174: 4301-6 (2005)
Active kinase proteome screening reveals novel signal complexity in cardiomyopathy
Fernando, Pasan, et al (2005) Mol Cell Proteomics, 4:673-82 (2005)
p38 Mitogen-activated protein kinase-dependent and -independent signaling of mRNA stability of AU-rich element-containing transcripts
Frevel, Mathias A E, et al (2003) Mol Cell Biol, 23:425-36 (2003)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R IP, WB Rabbit Purified Monoclonal Antibody
Description:
Anti-MKK6 NT Antibody, rabbit monoclonal
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Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes MKK6.
Molecular Weight:
36 kDa
Epitope:
N-terminus
Immunogen:
KLH-conjugated synthetic peptide corresponding to residues in the N-term of human MKK6.
Isotype:
IgG
Species Reactivity:
Human
Mouse
Rat
Application Notes:
Western Blot: 1:10,000.
Immunoprecipitation: 1:50. The use of protein G agarose is recommended.
Optimal working dilutions must be determined by the end user.
Control:
POSITIVE: HeLa cells.
Quality Assurance:
Routinely evaluated by immunoblot analysis.
Purification Method:
Protein G purfied
Presentation:
Rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
Storage Conditions:
2 years at -20°C from date of shipment.
UniProt Number:
P52564
Entrez Gene Number:
NM_002758.3
Gene Symbol:
MAP2K6
MEK6
PRKMK6
SAPKK3
MAPKK6
MKK6
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Alternate Names:
MAPKK6, MEK6, MKK6, PRKMK6, SAPKK3
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.
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UniProt Summary:
FUNCTION: SwissProt: P52564 # Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
SIZE: 334 amino acids; 37492 Da
SUBUNIT: Interacts with Yersinia yopJ.
TISSUE SPECIFICITY: Isoform 2 is only expressed in skeletal muscle. Isoform 1, on the other hand, is found in skeletal muscle, heart, and in lesser extent in liver or pancreas.
PTM: Weakly autophosphorylated. & Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathwa |