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Anti-MKK4 CT Antibody, rabbit monoclonal
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REFERENCES
A scanning peptide array approach uncovers association sites within the JNK/beta arrestin signalling complex.
Xiang Li,Ruth MacLeod,Allan J Dunlop,Helen V Edwards,Noopur Advant,Lucien C D Gibson,Nicola M Devine,Kim M Brown,David R Adams,Miles D Houslay,George S Baillie (2009) FEBS letters.583
Reduced metastasis-suppressor gene mRNA-expression in breast cancer brain metastases
Stark, Andreas M, et al (2005) J Cancer Res Clin Oncol, 131:191-8 (2005)
Ser/Thr protein phosphatase 5 inactivates hypoxia-induced activation of an apoptosis signal-regulating kinase 1/MKK-4/JNK signaling cascade
Zhou, Guofei, et al (2004) J Biol Chem, 279:46595-605 (2004)
A docking site in MKK4 mediates high affinity binding to JNK MAPKs and competes with similar docking sites in JNK substrates
Ho, David T, et al (2003) J Biol Chem, 278:32662-72 (2003)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R FC, IH(P), IP, WB Rabbit Purified Monoclonal Antibody
Description:
Anti-MKK4 CT Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes MKK4.
Molecular Weight:
44 kDa
Epitope:
C-terminus
Immunogen:
KLH-conjugated synthetic peptide corresponding to residues in the C-term of human MKK4.
Isotype:
IgG
Species Reactivity:
Human
Mouse
Rat
Application Notes:
Western Blot: 1:1000.
Immunoprecipitation: 1:50. The use of protein G agarose is recommended.
Flow Cytometry: 1:50.
Immunohistochemistry: 1:50-1:100 on paraffin-embeddedsections of human lung adenocarcinoma.
Optimal working dilutions must be determined by the end user.
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Quality Assurance:
Routinely evaluated by immunoblot analysis.
Purification Method:
Protein G purfied
Presentation:
Rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
Storage Conditions:
2 years at -20°C from date of shipment.
UniProt Number:
P45985
Entrez Gene Number:
NM_003010.2
Gene Symbol:
MAP2K4
SERK1
MAPKK4
JNKK1
SEK1
PRKMK4
MEK4
MKK4
JNKK
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Alternate Names:
JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SEK1, SERK1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.
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UniProt Summary:
FUNCTION: SwissProt: P45985 # Dual specificity kinase that acti |