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Anti-MKK3 NT Antibody, rabbit monoclonal
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REFERENCES
Regulation of p38 MAPK by MAPK kinases 3 and 6 in fibroblast-like synoviocytes.
Inoue, Tomoyuki, et al. (2005) J. Immunol., 174: 4301-6 (2005)
p38 kinase-mediated transactivation of the epidermal growth factor receptor is required for dedifferentiation of renal epithelial cells after oxidant injury
Zhuang, Shougang, et al (2005) J Biol Chem, 280:21036-42 (2005)
Comparative studies of a new subfamily of human Ste20-like kinases: homodimerization, subcellular localization, and selective activation of MKK3 and p38
Yustein, Jason T, et al (2003) Oncogene, 22:6129-41 (2003)
Species Reactivity Key Applications Host Format Antibody Type
H FC, IP, WB Rabbit Purified Monoclonal Antibody
Description:
Anti-MKK3 NT Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes MKK3
Molecular Weight:
40 kDa
Epitope:
N-terminus
Immunogen:
KLH-conjugated synthetic peptide corresponding to residues in the N-term of human MKK3.
Isotype:
IgG
Species Reactivity:
Human
Application Notes:
Western Blot: 1,5,000-1:20,000.
Immunoprecipitation: 1:50. The use of protein G agarose is recommended.
Flow Cytometry: 1:50.
Optimal working dilutions must be determined by the end user.
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Control:
POSITIVE: Jurkat, HeLa, MCF-7 or K562 cells.
Quality Assurance:
Routinely evaluated by immunoblot analysis.
Purification Method:
Protein G purfied
Presentation:
Rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
Storage Conditions:
2 years at -20°C from date of shipment.
UniProt Number:
P46734
Entrez Gene Number:
NM_145110.1
NM_002756.3
NM_145109.2
Gene Symbol:
MAP2K3
MAPKK3
PRKMK3
MEK3
MKK3
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Alternate Names:
MAPKK3, MEK3, PRKMK3
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.
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UniProt Summary:
FUNCTION: SwissProt: P46734 # Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP k |