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Anti-MEKK2 CT Antibody, rabbit monoclonal
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REFERENCES
Mip1, an MEKK2-interacting protein, controls MEKK2 dimerization and activation
Cheng, Jinke, et al (2005) Mol Cell Biol, 25:5955-64 (2005)
Regulation of c-Jun N-terminal kinase by MEKK-2 and mitogen-activated protein kinase kinase kinases in rheumatoid arthritis
Hammaker, Deepa R, et al (2004) J Immunol, 172:1612-8 (2004)
MEK5 and ERK5 are localized in the nuclei of resting as well as stimulated cells, while MEKK2 translocates from the cytosol to the nucleus upon stimulation
Raviv, Ziv, et al (2004) J Cell Sci, 117:1773-84 (2004)
Species Reactivity Key Applications Host Format Antibody Type
H, not M, not R FC, ICC, IH(P), IP, WB Rabbit Purified Monoclonal Antibody
Description:
Anti-MEKK2 CT Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes the C-terminal domain of MEKK2.
Molecular Weight:
70 kDa
Epitope:
C-terminus
Immunogen:
KLH-conjugated synthetic peptide corresponding to the C-terminal region of human MEKK2.
Isotype:
IgG
Species Reactivity:
Human
Non-Reactive Species:
Mouse
Rat
Application Notes:
Wetsren Blot: 1:1000.
Immunoprecipitation: 1:50. The use of protein G is recommended.
Flow Cytometry: 1:50.
Immunohistochemistry: 1:50 on paraffin embedded tissue sections of human breast carcinoma.
Immunofluorescence: 1:50-1:100. Paraformaldehyde fixation is recommended.
Optimal working dilutions must be determined by the end user.
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Quality Assurance:
Routinely evaluated by immunoblot analysis.
Purification Method:
Protein G purfied
Presentation:
Rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 90mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
Storage Conditions:
1 year at -20°C from date of shipment.
UniProt Number:
Q9Y2U5
Entrez Gene Number:
NM_006609.3
Gene Symbol:
MAP3K2
MEKK2B
MAPKKK2
MEKK2
Alternate Names:
MAP3K2, MEKK2B
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
Immunocytochemistry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Western Blotting
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Entrez Gene Summary:
The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process.
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UniProt Summary:
FUNCTION: SwissProt: Q9Y2U5 # Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7 (By similarity). Plays a role in caveolae kiss-and-run dynamics.
COFACTOR: Magnesium.
SIZE: 619 amino acids; 69741 Da
SUBUNIT: Binds both upstream activators and downstream substrat |