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Anti-phospho-EGFR (Tyr1173) Antibody, rabbit monoclonal
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REFERENCES
Requirement of Tyr-992 and Tyr-1173 in phosphorylation of the epidermal growth factor receptor by ionizing radiation and modulation by SHP2
Sturla, Lisa-Marie, et al (2005) J Biol Chem, 280:14597-604 (2005)
Integrin-induced epidermal growth factor (EGF) receptor activation requires c-Src and p130Cas and leads to phosphorylation of specific EGF receptor tyrosines
Moro, Laura, et al (2002) J Biol Chem, 277:9405-14 (2002)
Species Reactivity Key Applications Host Format Antibody Type
Human Only IF, IH(P), IP, WB Rabbit Purified Monoclonal Antibody
Description:
Anti-phospho-EGFR (Tyr1173) Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes human EGFR phosphorylated on tyrosine residue 1173.
Molecular Weight:
170 kDa
Immunogen:
KLH-conjugated synthetic peptide corresponding to the amino acid residues including and surrounding tyrosine 1173 of the human EGF Receptor (EGFR).
Modifications:
Phosphorylation
Isotype:
IgG
Species Reactivity:
Human Only
Quality Assurance:
Routinely evaluated by immunoblot.
Presentation:
In 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Storage Conditions:
2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
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UniProt Number:
P00533
Entrez Gene Number:
NM_005228.3
NM_201282.1
NM_201283.1
NM_201284.1
Gene Symbol:
EGFR
ERBB1
ERBB
mENA
Alternate Names:
ERBB, ERBB1, epidermal growth factor receptor
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunofluorescence
Immunohistochemistry (Paraffin)
Immunoprecipitation
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
SIZE: 1210 amino acids; 134277 Da
SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are as |