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Anti-EGFR Antibody, rabbit monoclonal
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REFERENCES
Prostaglandin E2 regulates cell migration via the intracellular activation of the epidermal growth factor receptor.
Buchanan, F Gregory, et al. (2003) J. Biol. Chem., 278: 35451-7 (2003)
Integration of growth factor, extracellular matrix, and retinoid signals during bronchial epithelial cell differentiation.
Moghal, N and Neel, B G (1998) Mol. Cell. Biol., 18: 6666-78 (1998)
Species Reactivity Key Applications Host Format Antibody Type
H, not M, not R FC, IF, IP, WB Rabbit Purified Monoclonal Antibody
Description:
Anti-EGFR Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes the C-terminal domain of EGFR and does not cross-react with any other ErbB family member.
Molecular Weight:
170 kDa
Immunogen:
KLH-conjugated synthetic peptide corresponding to the C-terminal region of human EGF Receptor (EGFR).
Isotype:
IgG
Species Reactivity:
Human
Non-Reactive Species:
Mouse
Rat
Application Notes:
Western Blot: 1:10,000 on lysates from A431 cells.
Immunoprecipitation: 1:30.
Flow Cytometry: 1:10. Permeablization of cells required.
Immunofluorescence: 1:50. Paraformaldehyde fixation recommended.
Optimal working dilutions must be determined by the end user.
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Control:
POSITIVE: A431 cells.
Quality Assurance:
Routinely evaluated by immunoblot analysis.
Presentation:
Rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
Storage Conditions:
2 years at -20°C from date of shipment.
UniProt Number:
P00533
Entrez Gene Number:
NM_005228.3
NM_201282.1
NM_201283.1
NM_201284.1
Gene Symbol:
EGFR
ERBB1
ERBB
mENA
Alternate Names:
Epidermal growth factor receptor; ERBB; ERBB1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
Immunofluorescence
Immunoprecipitation
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
SIZE: 1210 amino acids; 134277 Da
SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
SIMILARITY: SwissProt: P00533 ## Belongs to the prote |