|
Anti-phospho-eIF-2α (Ser51) Antibody, rabbit monoclonal
Close
REFERENCES
Persistent eIF2alpha(P) is colocalized with cytoplasmic cytochrome c in vulnerable hippocampal neurons after 4 hours of reperfusion following 10-minute complete brain ischemia.
Page, Andrea B, et al. (2003) Acta Neuropathol., 106: 8-16 (2003)
Proteasome inhibitor PS-341 induces apoptosis in cisplatin-resistant squamous cell carcinoma cells by induction of Noxa
Fribley, Andrew M, et al (2006) J Biol Chem, 281:31440-7 (2006)
Double-stranded RNA-dependent protein kinase phosphorylation of the alpha-subunit of eukaryotic translation initiation factor 2 mediates apoptosis
Scheuner, Donalyn, et al (2006) J Biol Chem, 281:21458-68 (2006)
Tyrosine phosphorylation acts as a molecular switch to full-scale activation of the eIF2alpha RNA-dependent protein kinase
Su, Qiaozhu, et al (2006) Proc Natl Acad Sci USA, 103:63-8 (2006)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R ICC, IH(P), WB Rabbit Purified Monoclonal Antibody
Description:
Anti-phospho-eIF-2α (Ser51) Antibody, rabbit monoclonal
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes the eIF-2α when phosphorylated on Ser51.
Molecular Weight:
36 kDa
Immunogen:
KLH-conjugated synthetic phospho-peptide corresponding to residues surrounding Ser51 of human eIF-2α
Modifications:
Phosphorylation
Isotype:
IgG
Species Reactivity:
Human
Mouse
Rat
Application Notes:
Suggested Dilutions:
Immunoblot Analysis: 1:1,000
Immunocytochemistry: 1:100 - 1:250
Immunohistochemistry: 1:50-1:100
Quality Assurance:
Routinely evaluated by immunoblot.
Purification Method:
Serum
Presentation:
100 μL of rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
Storage Conditions:
2 years at -20°C from date of shipment.
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
View All »
UniProt Number:
Q9BY44
Entrez Gene Number:
NM_032025.3
Gene Symbol:
EIF2A
eIF-2A
MSTP089
MSTP004
MST089
EIF-2A
ORFNames=CDA02
CDA02
View All »
Alternate Names:
EIF-2A
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Immunocytochemistry
Immunohistochemistry (Paraffin)
Western Blotting
Entrez Gene Summary:
EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al., 2002 [PubMed 12133843]).[supplied by OMIM]
UniProt Summary:
FUNCTION: SwissProt: Q9BY44 # Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF- 2 |