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Anti-phospho-Cbl (Tyr774) Antibody, rabbit monoclonal
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REFERENCES
Fyn, Yes, and Syk phosphorylation sites in c-Cbl map to the same tyrosine residues that become phosphorylated in activated T cells
Feshchenko, E A, et al (1998) J Biol Chem, 273:8323-31 (1998)
APS facilitates c-Cbl tyrosine phosphorylation and GLUT4 translocation in response to insulin in 3T3-L1 adipocytes
Liu, Jun, et al (2002) Mol Cell Biol, 22:3599-609 (2002)
Species Reactivity Key Applications Host Format Antibody Type
H, not M, not R FC, IF, IP, WB, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-phospho-Cbl (Tyr774) Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Cbl when phosphorylated on tyrosine 774.
Molecular Weight:
120 kDa
Immunogen:
KLH-conjugated synthetic peptide corresponding to the amino acid residues surrounding tyrosine 774 of human Cbl.
Modifications:
Phosphorylation
Isotype:
IgG
Species Reactivity:
Human
Non-Reactive Species:
Mouse
Rat
Application Notes:
Western Blot: 1:100 on lysates from pervanadate-treated Jurkat cells.
Immunoprecipitation: 1:20.
Flow Cytometry: 1:20. Permeablization of cells is required.
Immunohistochemistry: 1:25 on paraffin-embedded sections of human cervical carcinoma.
Immunofluorescence: 1:25. Paraformaldehyde fixation recommended.
Optimal working dilutions must be determined by the end user.
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Control:
POSITIVE: Pervanadate-treated Jurkat cells.
Quality Assurance:
Routinely evaluated by immunoblot analysis.
Presentation:
Rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
Storage Conditions:
2 years at -20°C from date of shipment.
UniProt Number:
P22681
Entrez Gene Number:
NM_005188.2
Gene Symbol:
CBL
CBL2
RNF55
C-CBL
Alternate Names:
Cas-Br-M (murine) ecotropic retroviral transforming sequence
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
Immunofluorescence
Immunoprecipitation
Western Blotting
Immunohistochemistry (Paraffin)
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Entrez Gene Summary:
The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation.
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UniProt Summary:
FUNCTION: SwissProt: P22681 # Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated rece |