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Anti-EGFR (CT) Antibody, clone 13G8
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REFERENCES
The angiogenic factor angiopoietin-1 is a proneurogenic peptide on subventricular zone stem/progenitor cells.
Alexandra I Rosa,Joana Gonçalves,Luísa Cortes,Liliana Bernardino,João O Malva,Fabienne Agasse (2010) The Journal of neuroscience : the official journal of the Society for Neuroscience.30
Somatic mutations of the epidermal growth factor receptor and non-small-cell lung cancer.
Zhang, Xiaozhu and Chang, Alex (2007) J. Med. Genet., 44: 166-72 (2007)
Gefitinib induces premature senescence in non-small cell lung cancer cells with or without EGFR gene mutation.
Hotta, Katsuyuki, et al. (2007) Oncol. Rep., 17: 313-7 (2007)
Identification of a novel EGF-sensitive cell cycle checkpoint
Walker, Francesca, et al (2007) Exp Cell Res, 313:511-26 (2007)
Species Reactivity Key Applications Host Format Antibody Type
H, M ELISA, ICC, WB Mouse Purified Monoclonal Antibody
Description:
Anti-EGFR (CT) Antibody, clone 13G8
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Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes C-terminus domain of EGF receptor (aa1165-1186)
Molecular Weight:
180 kDa
Immunogen:
KLH-conjugated synthetic peptide encompassing the surrounding amino acids of the C terminus in human EGFR
Clone:
13G8
Isotype:
IgG1
Species Reactivity:
Human
Mouse
Control:
Includes EGF treated Hep2G lysate as a positive control
Quality Assurance:
Routinely evaluated by immunoblot.
Purification Method:
Subsequent thiophilic adsorption and size exclusion chromatography
Presentation:
100 µg of purified mouse monoclonal IgG lyophilized in 2X PBS containing 0.09% sodium azide, PEG, and sucrose
Storage Conditions:
2 years at -20°C from date of shipment
UniProt Number:
P00533
Entrez Gene Number:
NM_005228.3
NM_201282.1
NM_201283.1
NM_201284.1
Gene Symbol:
EGFR
ERBB1
ERBB
mENA
Alternate Names:
ERBB; mENA; ERBB1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
ELISA
Immunocytochemistry
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
SIZE: 1210 amino acids; 134277 Da
SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung ca |