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Anti-erbB2 (intracellular domain) Antibody, clone 24B5
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REFERENCES
Removal of the membrane-anchoring domain of epidermal growth factor leads to intracrine signaling and disruption of mammary epithelial cell organization.
Wiley, H S, et al. (1998) J. Cell Biol., 143: 1317-28 (1998)
Epidermal growth factor receptor inhibition strategies in pancreatic cancer: past, present and the future
Cohenuram, Michael and Saif, Muhammad Wasif (2007) JOP, 8:4-15 (2007)
Species Reactivity Key Applications Host Format Antibody Type
H, M ELISA, WB Mouse Purified Monoclonal Antibody
Description:
Anti-erbB2 (intracellular domain) Antibody, clone 24B5
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes the intracellular domain of erbB2 (aa 860-880).
Molecular Weight:
185 kDa
Immunogen:
KLH-conjugated synthetic peptide encompassing the surrounding amino acids of the intracellular domain in human erbB2
Clone:
24B5
Isotype:
IgG
Species Reactivity:
Human
Mouse
Control:
EGF treated SKOV-3 lysates
Quality Assurance:
Routinely evaluated by immunoblot.
Purification Method:
Subsequent thiophilic adsorption and size exclusion chromatography
Presentation:
lyophilized in 2X PBS containing 0.09% sodium azide, PEG, and sucrose.
Storage Conditions:
2 years at -20°C from date of shipment. Reconstitute prior to use. Reconstitute with 1 mL H2O for 15 min at room temperature. Reconstituted aliquots should be stored frozen at -80°C. Thawed aliquots may be stored at 4°C for up to 3 months. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
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UniProt Number:
P04626
Entrez Gene Number:
NM_001005862.1
NM_004448.2
Gene Symbol:
ERBB2
HER2
HER-2
NGL
herstatin
erbB-2
C-erbB-2
NEU
TKR1
p185erbB2
HER-2/neu
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Alternate Names:
ERBB2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
ELISA
Western Blotting
Entrez Gene Summary:
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
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UniProt Summary:
FUNCTION: SwissProt: P04626 # Essential component of a neuregulin-receptor complex, although neuregulins |